Establishment and characterization of a cell line derived from fin of the endangered Yangtze sturgeon ( Acipenser dabrya
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Establishment and characterization of a cell line derived from fin of the endangered Yangtze sturgeon (Acipenser dabryanus) Juanjuan Liu 1,2
&
Xueqing Liu 1,2 & Qingkai Zeng 1,2 & Binzhong Wang 1,2 & Kan Xiao 1,2 & Chun Tan 1,2 & Hejun Du 1,2
Received: 23 June 2020 / Accepted: 28 July 2020 / Editor: Tetsuji Okamoto # The Society for In Vitro Biology 2020
Abstract Yangtze sturgeon (Acipenser dabryanus) is an endangered endemic freshwater fish of China. Cell-line is a potential means used for long-term preservation of germplasm resources and an ideal in vitro model in place of living organisms for biological studies. Here, culture condition and characterization of fin-derived cell in Yangtze sturgeon were carried out. Tissue explant techniques have been efficiently used in the Yangtze sturgeon caudal fin (YSCF) culture. The YSCF cell line showed a fibroblast-like morphology and stable growth in minimum essential medium eagle’s (MEME) supplemented with 10–20% fetal bovine serum at 25°C. Cells were cryopreserved with preservative DMSO in liquid nitrogen and grew normally after recovery. No bacterial, fungal, or mycoplasma contamination was detected in the YSCF cells. Karyotype analysis of the YSCF cells showed that the chromosome numbers of the YSCF ranged from 242 to 273, and the modal chromosome number was identified as 264 at passage 9. The YSCF cells were confirmed from A. dabryanus by assay of 16S rRNA and COI. Furthermore, GFP reporter gene was successfully transferred into YSCF cells and expressed. The established YSCF cell lines will contribute to the preservation of germplasm resources and provide a useful vitro tool for further biological studies in sturgeon species. Keywords Acipenser dabryanus . Cell lines . Chromosome analysisSigma . Primary cell culture
Introduction Yangtze sturgeon, Acipenser dabryanus, or Dabry’s sturgeon, is one of three endemic sturgeon species in the Yangtze River, mainly distributed in the upper main stream of the Yangtze River and its tributaries (YARSG 1988; Zhuang et al. 1997). Its population declined dramatically over the past three decades because of overfishing, environmental pollution, habitat degradation, and dam construction. Since 1990, no natural reproduction of the species has been found (Wan et al. 2003; Zhang et al. 2011). Then, it was listed as a critically endangered species in the International Union for Conservation of Nature Red List in 2010 (IUCN 2010). Despite that multiple conservation strategies have been taken to recover the species, * Hejun Du [email protected] Juanjuan Liu [email protected] 1
Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang 443100, Hubei, China
2
China Three Gorges Corporation, Chinese Sturgeon Research Institute, Yichang 443100, Hubei, China
such as habitat restoration, artificial propagation, and public education in coordination with national recovery policy and regulation, the wild population has been basically extinct, only a few dozen wild individuals remain in artificial conservation. T
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