Isolation, establishment and characterization of new insect cell line derived from midgut of rice weevil, Sitophilus ory
- PDF / 346,274 Bytes
- 5 Pages / 595.276 x 790.866 pts Page_size
- 21 Downloads / 187 Views
REPORT
Isolation, establishment and characterization of new insect cell line derived from midgut of rice weevil, Sitophilus oryzae Bhumi Thakkar 1 & Parth Pandya 2 & Pragna Parikh 1 Received: 4 May 2020 / Accepted: 19 October 2020 / Editor: Tetsuji Okamoto # The Society for In Vitro Biology 2020
The development of insect cell lines has been the subject of intense research since the 1980s for industrial production of proteins, vaccines, and insecticides (Drugmand et al. 2012). During the last four decades, research about insect cell culture has revealed promising outcomes, leading to the development of high-speed screening technologies as new insect pest management tools (Airenne et al. 2013). Until now, insect cell lines have been derived from embryos, ovaries, hemocytes, testis, newly hatched larvae (neonate larvae), pupae, fat body, silk glands, and epidermis (Hu et al. 2014; Zhang et al. 2014; Zheng et al. 2014). The above literature describes the development of cell lines from agricultural pests, while the development of cell lines from stored grain pests remains elusive. Moreover, there is a gap in the detailed studies of insect cell lines derived from Coleoptera in general and stored grain pest in particular. Until now, cell lines from only eight species of coleopteran insects belonging to the families Scarabaeidae (Mitsuhashi 2003), Chrysomelidae (Charpentier et al. 2002; Long et al. 2002), Curculionidae (Aljabr et al. 2014; Zheng et al. 2014), Tenebrionidae (Goodman et al. 2012), and Cerambycidae (Hoshino et al. 2009) have been established. Nevertheless, the continued development of new cell cultures Supplementary Information The online version contains supplementary material available at https://doi.org/10.1007/s11626-02000523-9. * Pragna Parikh [email protected] Bhumi Thakkar [email protected] Parth Pandya [email protected] 1
Division of Entomology, Department of Zoology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat 390002, India
2
Division of Biomedical and Life science, School of Science, Navrachana University, Vadodara, India
from other species is vital for the growth of insect cell studies. By keeping these studies in view, the present study was focused on developing, characterizing, and establishing a new cell line from the midgut tissue of the insect pest adult S. oryzae. Adult S. oryzae (4.28 ± 0.76 mm) was collected from the stored grain warehouse of Anand, Gujarat. The insects were identified using standard taxonomical keys. Simultaneously, they were kept in a small jar containing pesticide-free grains for mass production. All insect cultures were maintained at standard laboratory conditions (Thakkar and Parikh 2018). All procedures were carried out in a laminar flow hood (Labtach, Ahmedabad, India). For each explant of midgut cells, approximately 40 to 50 insects were separated and starved for 2 to 3 h prior to the isolation of cells. Surface sterilization was done by immersing the insects each in a mixture of aqueous solution (20% septisol + 1%
Data Loading...
