Establishment of consomic strains derived from A/J and SM/J mice for genetic analysis of complex traits
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Establishment of consomic strains derived from A/J and SM/J mice for genetic analysis of complex traits Tamio Ohno • Keiko Hata • Taisuke Baba • Fusayo Io • Misato Kobayashi • Fumihiko Horio Masahiko Nishimura
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Received: 27 June 2012 / Accepted: 7 September 2012 / Published online: 10 October 2012 Ó Springer Science+Business Media New York 2012
Abstract Consomic strains, in which one chromosome is derived from a donor strain and the other chromosomes are derived from the recipient strain, provide a powerful tool for the dissection of complex genetic traits. In this study we established ten consomic strains (A-2SM, A-6SM, A-11SM, A-12SM, A-13SM, A-15SM, A-17SM, A-18SM, A-19SM, A-YSM) using the SM/J strain as the donor and the A/J strain as the recipient; these are the parental strains of a set of SMXA recombinant inbred (RI) strains that we had developed previously. We analyzed body weights and blood lipid levels in the consomic and parental strains. The mean values for each trait showed a continuous range of variation in the consomic strains suggesting that they are controlled by multiple genes. We previously identified suggestive QTLs for body weight on chromosome 6 in SMXA RI strains and (SM/J 9 A/J)F2 mice. The observation that the A-6SM consomic strain had a significantly lower mean body weight than the A/J strain supports the presence of this QTL on chromosome 6. Similarly, the higher blood triglyceride level in the A-11SM strain shows the existence of a previously mapped QTL on chromosome 11, and the A-12SM strain provides evidence of a QTL for blood total cholesterol level on chromosome 12. These consomic strains, along with the previously developed set of SMXA RI strains from A/J and SM/J mice, offer an
T. Ohno (&) K. Hata T. Baba M. Nishimura Division of Experimental Animals, Graduate School of Medicine, Nagoya University, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan e-mail: [email protected] F. Io M. Kobayashi F. Horio Department of Applied Molecular Bioscience, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan
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invaluable and powerful resource for the analysis of complex genetic traits in mice.
Introduction Recombinant inbred (RI) strains are derived from the F2 generation of a cross between parental strains; random pairs of F2 mice are mated and the line is maintained in subsequent generations by brother–sister mating. The chromosomes in each RI strain are a genetically unique mixture of those of the parental strains. A set of RI strains provides a valuable resource for the chromosomal mapping of complex genetic traits (Darvasi 1998; Brockmann and Bevova 2002). RI strains are maintained as isogenic lines; therefore, a number of genetically identical individuals can be produced for phenotypic analysis. As phenotypic values for each strain are calculated as the mean value of many genetically identical mice, those of RI strains more closely reflect the genotype than those of crossbred populations, which is advantageous
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