Evaluation and QTL Mapping of Salt Tolerance in Yardlong Bean [ Vigna unguiculata (L.) Walp. Subsp. unguiculata Sesquipe
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ORIGINAL PAPER
Evaluation and QTL Mapping of Salt Tolerance in Yardlong Bean [Vigna unguiculata (L.) Walp. Subsp. unguiculata Sesquipedalis Group] Seedlings Hongmei Zhang 1,2 & Wenjing Xu 2,3 & Huatao Chen 2 & Jingbin Chen 2 & Xin Chen 2 & Shouping Yang 1
# Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Salinity is a major abiotic stress that limits productivity in yardlong bean. Identification of the quantitative trait loci (QTL) underlying salt tolerance is a prerequisite for successful marker-assisted selection when breeding for salt-tolerance. The objective of this study was to use a set of insertions and deletions (InDels) found in salt stress-responsive genes to construct a linkage map and identify reliable markers that can guide breeding efforts for novel, high-yield, salttolerant varieties of yardlong bean (Vigna unguiculata). A linkage map was constructed from 103 InDel markers in an F2 population consisting of 143 plants produced by crossing yardlong bean varieties Suzi 41 (salt tolerant) and Sujiang 1419 (salt sensitive). The markers were clustered across 9 chromosomes spanning 975.7 cM in total length, with an average distance between adjacent markers of 9.5 cM. Using this linkage map, we identified six major QTLs associated with control of salt tolerance-related traits on chromosomes (Chr.) 08, 09, and 11, which explained 9.9% ~ 72.7% of the phenotypic variation. Four major QTLs showed co-location in the region between VUIn584 and VUIn724 on Chr.09, associated with the traits of salt tolerance rating (STR), leaf chlorophyll content (SPAD), aerial biomass fresh weight (AFW), and root fresh weight (RFW). These QTLs provide a valuable reference for marker-assisted breeding, QTL fine mapping, and the cloning and characterization of salt-tolerance candidate genes. Keywords Genetic map . InDel marker . QTL analysis . Salt stress . Vigna unguiculata
Key message In this study, we constructed a linkage map from InDel markers. Using this linkage map, we identified four major QTLs associated with salt tolerance-related traits in the region between markers VUIn584 and VUIn724 on Chr.09, and a major QTL on Chr.08 and 11, respectively. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11105-020-01194-2) contains supplementary material, which is available to authorized users. * Shouping Yang [email protected] Hongmei Zhang [email protected]
Xin Chen [email protected] 1
Soybean Research Institute of Nanjing Agricultural University/ National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, Jiangsu, China
Huatao Chen [email protected]
2
Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, No. 50, Zhongling Street, Nanjing 210014, Jiangsu, China
Jingbin Chen [email protected]
3
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China
Wenjing Xu [email protected]
Plant Mol Biol Rep
Abbreviations STR Salt tolerance rating SPAD The le
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