A Putative Common Bean Chalcone O -Methyltransferase Improves Salt Tolerance in Transgenic Arabidopsis thaliana
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A Putative Common Bean Chalcone O‑Methyltransferase Improves Salt Tolerance in Transgenic Arabidopsis thaliana Harun Niron1 · Müge Türet1 Received: 29 April 2019 / Accepted: 7 October 2019 © Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract Resolving the mechanism of salt stress tolerance in plants becomes a necessity to eventually develop salt-tolerant varieties by determining the key players in tolerance mechanism. Our previous results on transcriptome analysis of common bean under salt stress have revealed an upregulation in a putative chalconeO-methyltransferase (pvChOMT) transcript. In the present study, overexpression of pvChOMT gene in Arabidopsis transgenic lines enhanced salt tolerance. In turn, transgenic plants presented enhanced root growth, better water content, better mass preservation with osmoprotectant accumulation, and higher germination rates in saline conditions. Alignment analysis and predicted structure of pvChOMT have implicated it as the homologue of Medicago sativa chalcone O-methyltransferase (1FP1) with same substrate specificity. Promotor analysis of pvChOMT has shown a TATA box at − 145 position putative cis-acting elements within 1.5-kb upstream of the gene known to be activated through ABA-dependent and ABA-independent manner. Our results suggest that pvChOMT can be a good candidate gene to improve crops for salt stress tolerance. Keywords Phaseolus vulgaris L. · Salt stress tolerance · O-methyltransferase · Common bean · Transgenic Arabidopsis plant · Overexpression
Introduction Soil salinity and salt stress have become an important threat to agriculture. A soil with an electrical conductivity (EC) of 4dS/m (approx. 40 mM NaCl) is considered as saline (Shrivastava and Kumar 2015). Salt abundance on Earth creates a major environmental constraint for agricultural productivity on which the sustainability of human population depends (Brown et al. 1987). Nearly 20% of the cultivated and 33% of irrigated agricultural lands are estimated to be affected by excessive salinity. Moreover, these rates can increase roughly by 10% per annum due to various causes, such as Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00344-019-10040-z) contains supplementary material, which is available to authorized users. * Harun Niron [email protected] Müge Türet [email protected] 1
Department of Molecular Biology and Genetics, Bogazici University, Istanbul, Turkey
low rainfall, high evaporation, irrigation with salt-rich water, or inadequate agricultural methodology. Projections have indicated that at least 50% of the cultivable land to be saline by 2050 (Jamil et al. 2011). Common bean (Phaseolus vulgaris L.) is a glycophyte crop that accounts for approximately half of the consumed grain legumes globally (Broughton et al. 2003). However, even moderate salinity (1dS/m) can dramatically reduce the productivity of this legume (Chinnusamy et al. 2005). Drastic effects of salinity were observed in seed germination and see
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