Evaluation of the antimicrobial activity and compressive strength of a dental cement modified using plant extract mixtur
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B I O M A T E R I A L S S Y N T H E S I S A N D CH A R A C T E R I Z A T I O N Original Research
Evaluation of the antimicrobial activity and compressive strength of a dental cement modified using plant extract mixture Lamia Singer
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Gabriele Bierbaum2 Katja Kehl2 Christoph Bourauel3 ●
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Received: 20 January 2020 / Accepted: 27 October 2020 / Published online: 28 November 2020 © The Author(s) 2020
Abstract Literature lacks sufficient data regarding addition of natural antibacterial agents to glass ionomer cement (GICs). Hence, the aim of the study was to increase the antimicrobial properties of GICs through its modification with mixture of plant extracts to be evaluated along with an 0.5% chlorohexidine-modified GIC (CHX-GIC) with regard to biological and compressive strength properties. Conventional GIC (freeze-dried version) and CHX were used. Alcoholic extract of Salvadora persica, Olea europaea, and Ficus carcia leaves were prepared using a Soxhlet extractor for 12 h. The plant extract mixture (PE) was added in three different proportions to the water used for preparation of the dental cement (Group 1:1 PE, 2:1 PE, and 1:2 PE). Specimens were then prepared and tested against the unmodified GIC (control) and the 0.5% CHX-GIC. Chemical analysis of the extract mixture was performed using Gas chromatography–mass spectrometry. Antimicrobial activity was evaluated using agar diffusion assay against Micrococcus luteus and Streptoccocus mutans. Compressive strength was evaluated according to ISO 9917-1:2007 using a Zwick testing machine at a crosshead speed of 0.5 mm/min. Antimicrobial activity against Streptoccocus mutans was significantly increased for all the extract-modified materials compared to the unmodified cement, and the highest concentration was comparable to the CHX-GIC mixture. The activity against Micrococcus luteus was also significantly increased, but only for the material with the highest extract concentration, and here the CHX-GIC group showed statistically the highest antimicrobial activity. Compressive strength results revealed that there was no statistically significant difference between the different mixtures and the control except for the highest tested concentration that showed the highest mean values. The plant extracts (PEs) enhanced the antimicrobial activity against S. mutans and also against M. luteus in the higher concentration while compressive strength was improved by addition of the PE at higher concentrations. Graphical Abstract
* Lamia Singer [email protected] 1
Oral Technology, University Hospital Bonn, Bonn, Germany
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Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn, Germany
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Endowed Chair of Oral Technology, University Hospital Bonn, Bonn, Germany
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1 Introduction Glass ionomer cements (GICs) belong to a group of materials known as acid-base cements. The proper name for them, according to the International Organization for Standardization (ISO) is “glass polyalkenoate cem
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