Exogenous trehalose ameliorates methyl viologen induced oxidative stress through regulation of stomatal pore opening and
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RESEARCH ARTICLES
Exogenous trehalose ameliorates methyl viologen induced oxidative stress through regulation of stomatal pore opening and glutathione metabolism in tomato seedlings Pankaj Borgohain1 · Bhaben Chowardhara1 · Bedabrata Saha1,2 · Jay Prakash Awasthi1 · Preetom Regon1 · Smita Sahoo1 · Sanjib Kumar Panda1,3 Received: 22 September 2019 / Revised: 5 August 2020 / Accepted: 7 August 2020 © Society for Plant Research 2020
Abstract Oxidative burst is the common thread linking all abiotic and biotic stressors. So in this study non-reducing disaccharide trehalose (Tre) has been examined for attenuation of oxidative stress, imposed through methyl viologen (MV) foliar spray in tomato seedlings. Initially the ameliorating property was ascertained by leaf disc senescence and stomatal pore opening assay, before carrying out the foliar spray on seedlings. Both these assays showed Tre mediated mitigation of MV induced oxidative stress. It was also observed that trehalose dose determines the extent of stomatal pore opening. MV treated seedlings showed more reactive oxygen species (ROS) accumulation compared to control, Tre and MV + Tre treated plants both histochemically and quantitatively. Damage due to ROS accumulation was documented by quantifying malondialdehye and lipoxygenase activity. Finally, the response of the tomato seedlings to oxidative stress was monitored by quantifying the antioxidant enzyme activities and non-enzymatic antioxidant content under stressed and unstressed conditions. Also transcript levels of glutathione synthetase (GS) and gamma-glutamylcysteine synthetase (γ-ECS), enzymes responsible for GSH synthesis; and transcription factors no apical meristem ATAF and cup-shaped cotyledon (NAC2) and dehydrationresponsive element-binding (DREB) were found to increase on application of Tre. All of these experimentations showed better adaptability and survivability of the seedlings treated exogenously with both MV and Tre in comparison to those treated only with MV. keywords Trehalose · Methyl viologen · Oxidative stress · Tomato
Introduction Plants being non-motile, continuously encounter a wide range of harsh environmental conditions. Abiotic stresses including heat, cold, salinity and drought, adversely effect plant metabolism leading to reduced growth and development thus resulting in decreased agriculture productivity * Sanjib Kumar Panda [email protected] 1
Plant Molecular Biotechnology Lab, Department of Life Science and Bioinformatics, Assam University, Silchar 788011, India
2
Present Address: School of Biological Sciences, National Institute of Science Education and Research, Bhubaneswar, Jatani 752050, India
3
Present Address: Department of Biochemistry, Central University of Rajasthan, Ajmer 305817, India
which accounts for more than 50% yield loss worldwide every year (Bray et al. 2000; Lobell et al. 2011; Shao et al. 2009). Mostly stresses are interconnected through crosstalk of responses elicited, causing similar cellular damage but differing in intensity
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