Overexpression of the sweetpotato peroxidase gene swpa4 enhances tolerance to methyl viologen-mediated oxidative stress
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SHORT COMMUNICATION
Overexpression of the sweetpotato peroxidase gene swpa4 enhances tolerance to methyl viologen-mediated oxidative stress and dehydration in Arabidopsis thaliana Yun-Hee Kim1 • Jeum Kyu Hong2 • Ho Soo Kim3 • Sang-Soo Kwak3 Received: 12 January 2020 / Accepted: 18 August 2020 Ó Society for Plant Biochemistry and Biotechnology 2020
Abstract We previously reported that transgenic Arabidopsis thaliana plants overexpressing the sweet potato peroxidase gene swpa4 under the control of the cauliflower mosaic virus (CaMV) 35 s promoter showed increased levels of reactive oxygen species (ROS) and nitric oxide (NO), and higher expression of ROS and NO related genes than control plants. Here, we investigated the effect of swpa4 overexpression on the abiotic and biotic stress tolerance levels of Arabidopsis plants. Methyl viologen (MV) treatment-induced oxidative stress caused visible damage to the seedlings and rosette leaves of all Arabidopsis plants, although the symptoms were more severe in control plants than in transgenic lines. Additionally, survival rates and ion leakage showed a slight decline in transgenic lines but a more severe decline in control plants after MV treatment. Transgenic plants also showed enhanced tolerance to drought stress. Dehydration treatment, followed by rehydration, resulted in a greater change in the relative water content and lipid peroxidation of control plants than in that of transgenic lines. However, transgenic plants did not show enhanced resistance to biotic stresses such as bacterial and fungal pathogens. These results indicate that transgenic Arabidopsis plants can efficiently regulate defense levels during oxidative stress via the overexpression of swpa4. Keywords Dehydration Oxidative stress Peroxidase Reactive oxygen species Sweetpotato Transgenic arabidopsis Abbreviations MV Methyl viologen NO Nitric oxide PR Pathogenesis-related ROS Reactive oxygen species RWC Relative water contents
& Yun-Hee Kim [email protected] & Sang-Soo Kwak [email protected] 1
Department of Biology Education, College of Education, and IALS, Gyeongsang National University, Jinju 660-701, Korea
2
Department of Horticulture, Gyeongnam National University of Science & Technology, JinJu, Korea
3
Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Korea
In the natural environment, plants are subject to various abiotic and biotic stresses, leading to excess production of reactive oxygen species (ROS) (Baxter et al. 2014). To decrease the damage caused by ROS, plants activate the enzymatic system, which mainly comprises large families of peroxidases. Secretory peroxidases catalyze the reduction of hydrogen peroxide (H2O2) via electron transport to various donor molecules, such as phenolic compounds, lignin precursors, cell wall compounds, and auxin (Hiraga et al. 2001). These enzymes also play an important role in abiotic stress tolerance and pathogen resistance, as reported in various plant species including rice
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