Expression of a rice Zn transporter, OsZIP1 , increases Zn concentration in tobacco and finger millet transgenic plants
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ORIGINAL ARTICLE
Expression of a rice Zn transporter, OsZIP1, increases Zn concentration in tobacco and finger millet transgenic plants Yamunarani Ramegowda • Ramegowda Venkategowda • Pavithra Jagadish • Geetha Govind • Rajashekar-Reddy Hanumanthareddy • Udayakumar Makarla Shankar Ambarahalli Guligowda
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Received: 17 April 2012 / Accepted: 20 September 2012 Ó Korean Society for Plant Biotechnology and Springer Japan 2012
Abstract Zinc (Zn) deficiency is very widespread both from the plant and human nutrition perspective. One of the approaches to improve Zn in crop plants is by overexpression of Zn transporters such that plants can uptake and accumulate additional Zn added to soil. The ZIP family transporters generally contribute to Zn homeostasis in plants by regulating Zn transport into the cell. We overexpressed OsZIP1 in finger millet and model plant tobacco under the control of constitutive (35S) and endospermspecific (Bx17) promoters to study the improvement in Zn accumulation. The transcript analysis revealed the induction of ZIP1 in leaf and root tissue under Zn deprivation in finger millet cultivars. Ectopic expression of OsZIP1 in tobacco under CaMV35S (n35S) and Bx17 (nBx17) improved seed Zn concentration compared to untransformed wild-type plants. In addition, we successfully developed finger millet transgenic plants, f35S and fBx17, expressing the OsZIP1 under 35S (f35S) and Bx17 (fBx17)
Electronic supplementary material The online version of this article (doi:10.1007/s11816-012-0264-x) contains supplementary material, which is available to authorized users. Y. Ramegowda R. Venkategowda P. Jagadish G. Govind R.-R. Hanumanthareddy U. Makarla S. A. Guligowda (&) Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bangalore 560 065, Karnataka, India e-mail: [email protected] Present Address: R. Venkategowda Department of Crop, Soil and Environmental Sciences, University of Arkansas, Fayetteville, AR 72701, USA Present Address: G. Govind Leibniz-Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 03, 06466 Gatersleben, Germany
promoter, respectively. The transgenic plants accumulated significantly higher Zn in seeds compared to untransformed wild-type finger millet plants. Plants expressing the gene under Bx17 promoter accumulated more Zn in seed than 35S plants. Apart from Zn, finger millet transgenic plants also showed increased Mn content in seeds. Our results show the involvement of OsZIP1 in improving Zn concentration in tobacco and finger millet. Using endospermspecific promoter, it is possible to improve the seed Zn concentration in the edible part of cereals. Keywords Tobacco
Zn Transporter OsZIP1 Finger millet
Introduction Finger millet (Eleusine coracana (L.) Gaertn.) accounts for 8 % of the area and 11 % of production of all millets globally (Bennetzen et al. 2003). It is grown under a wide range of environments in over 4 million hectares worldwide and is a primary food for millions in the dry lands of East and Central Afri
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