Transgenic Plants Methods and Protocols
In 2010 the global area of transgenic crops reached 148 million hectares, an 87-fold increase since 1996, making it the most rapidly adopted technology in the history of modern agriculture. In Transgenic Plants: Methods and Protocols, Second Edi
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IN
MOLECULAR BIOLOGY™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Transgenic Plants Methods and Protocols Second Edition Edited by
Jim M. Dunwell School of Biological Sciences, University of Reading, Reading, UK
Andy C. Wetten School of Biological Sciences, University of Reading, Reading, UK
Editors Jim M. Dunwell School of Biological Sciences University of Reading Reading, UK
Andy C. Wetten School of Biological Sciences University of Reading Reading, UK
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-557-2 e-ISBN 978-1-61779-558-9 DOI 10.1007/978-1-61779-558-9 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2012930136 © Springer Science+Business Media, LLC 2012 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface In 2010, the global area of transgenic crops reached 148 million hectares, an 87-fold increase since 1996, the first year of planting. This makes such technology the most rapidly adopted one in the history of modern agriculture. This success has been based on the scientific foundation developed in many international laboratories since the 1980s, and it is the purpose of the present volume to provide access to the continuous improvements being made for the production and analysis of transgenic plants. Importantly, this volume is designed to complement and extend the information published in this same series Transgenic Plants: Methods and Protocols in 2005. The present collection of novel methods is divided into eight parts which cover a range of protocols for both model and crop species. The first part covers various methods for the selection and detection of transgenic plants, and this is followed by a series of sections that describe specific methods for algae and then for higher plants. These latter sections are divided into those devoted to monocots, with an emphasis on rice, and then to dicots such as plum, grape, and cotton. Part VI covers a rapidly growing area of research, namely, the specific targeting and directed silencing of plant genes. Many of these technologies will cause considerable debate among the various regulatory regime
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