Expression of aquaporin 5 and the AQP5 polymorphism A(-1364)C in association with peritumoral brain edema in meningioma
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CLINICAL STUDY
Expression of aquaporin 5 and the AQP5 polymorphism A(-1364)C in association with peritumoral brain edema in meningioma patients Nicole Lambertz • Nicolai El Hindy • Cordula Adler • Katharina Rump Michael Adamzik • Kathy Keyvani • Agnes Bankfalvi • Winfried Siffert I. Erol Sandalcioglu • Hagen S. Bachmann
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Received: 28 August 2012 / Accepted: 29 January 2013 / Published online: 8 February 2013 Ó Springer Science+Business Media New York 2013
Abstract Aquaporins (AQP) are a growing family of water-channel proteins, numbering 13 to date. Recent studies have reported AQP1 and AQP4 to be involved in the development and resorption of brain edemas of different origin. Other AQPs have also been detected in brain tissue, but their impact on brain edema remains to be shown. To evaluate a possible role of AQP5 in brain edema, we investigated the association of AQP5 expression and the functional AQP5 promoter polymorphism A(-1364)C with occurrence and intensity of peritumoral edema in meningioma patients. Peritumoral edema was classified in three degrees based on preoperative imaging in 89 meningioma patients treated at the University Hospital Essen between 2003 and 2006. AQP5 expression was assessed immunohistochemically in tumor tissue obtained during neurosurgical tumor resection. Genotypes of the
A(-1364)C polymorphism were determined using the ‘‘slowdown’’ polymerase chain reaction. Higher levels of AQP5 expression were significantly correlated with the AQP5-1364 AA genotype (P = 0.02). AQP5 expression was positively correlated with edema (P = 0.04). AQP5 genotypes were not significantly associated with the occurrence, but with the intensity of peritumoral brain edema (P = 0.04). In our cohort, 40 % of patients with grade I, 66.7 % with grade II, and 76.5 % with grade III edema possessed at least one A allele. Development and intensity of peritumoral edema in meningiomas are associated with AQP5 expression. The intensity of edema correlates with the AQP5 A(-1364)C genotype. This suggests AQP5 as an interesting new candidate involved in peritumoral brain edema in meningioma patients. Keywords Aquaporins Edema Meningioma Oncogene Polymorphism
N. Lambertz (&) N. E. Hindy C. Adler I. Erol Sandalcioglu Department of Neurosurgery, Westdeutsches Tumorzentrum WTZ, University Hospital Essen, Hufelandstr. 55, 45147 Essen, Germany e-mail: [email protected] K. Rump M. Adamzik Department of Anesthesiology, Westdeutsches Tumorzentrum WTZ, University Hospital Essen, Hufelandstr. 55, 45147 Essen, Germany K. Keyvani A. Bankfalvi Institute of Pathology and Neuropathology, Westdeutsches Tumorzentrum WTZ, University Hospital Essen, Hufelandstr. 55, 45147 Essen, Germany W. Siffert H. S. Bachmann Institute of Pharmacogenetics, Westdeutsches Tumorzentrum WTZ, University Hospital Essen, Hufelandstr. 55, 45147 Essen, Germany
Introduction Intracranial meningiomas are generally benign, slow growing tumors with a broad diversity of histopathological variants. They arise from the arachnoid cell lay
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