The expression of bitter taste receptor TAS2R38 in patients with chronic rhinosinusitis
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(2020) 68:26
ORIGINAL ARTICLE
The expression of bitter taste receptor TAS2R38 in patients with chronic rhinosinusitis Karolina Zborowska‑Piskadło1 · Małgorzata Stachowiak2 · Natalia Rusetska2 · Elżbieta Sarnowska2 · Janusz Siedlecki2 · Karolina Dżaman1,3 Received: 18 November 2019 / Accepted: 1 September 2020 © L. Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland 2020
Abstract Chronic rhinosinusitis (CRS) is a frequent disease with high social impact and multifactorial pathogenesis. Recently, the bitter taste receptor TAS2R38 has been described to play a role in upper airway innate mucosal defense. The aim was to determine the localization and expression of the TAS2R38 in the selected cell lines and tissue collected from patient suffered from CRS as well as to correlate the results with clinical data. Moreover, the purpose was the estimation of the TAS2R38 distribution changes during acute and CRS. Forty-two patients undergoing nasal surgery were enrolled in the study. The TAS2R38 expression was assessed in the collected tissues using immunohistochemistry and immunocytochemistry methods. The western blot analysis was performed on human cell lines HeLa, MCF7, MDA-MB-231 to assess the location of the TAS2R38 protein. Moreover, the HeLa cell line was used as a model of acute inflammation induces by lipopolysaccharide. Immunohistochemistry analysis displayed a statistically significant difference of TAS2R38 level in the patients with CRS compared to healthy control and was different in CRS with and without nasal polyps. The results showed the abundance of TAS2R38 receptor in the cell nucleus in patients with CRS and cell lines. The variance in TAS2R38 receptor expression in two CRS types suggests their different pathogenesis. The first time in literature, we confirmed the presence of plasma membrane TAS2R38 receptor in the cell nuclei in CRS as well as in cell lines, what strongly suggests the different than membrane TAS2R38 function. Keywords TAS2R38 · Chronic rhinosinusitis · Bitter taste receptor Abbreviations CRS Chronic rhinosinusitis CRSwNP Chronic rhinosinusitis with nasal polyps CRSsNP Chronic rhinosinusitis sine nasal polyps EPOS European position paper on rhinosinusitis IHC Immunohistochemistry ICC Immunocytochemistry Karolina Zborowska-Piskadło and Małgorzata Stachowiak contributed equally to this work. * Karolina Dżaman [email protected] 1
Department of Otolaryngology, Miedzyleski Hospital, Warsaw, Poland
2
Department of Molecular and Translational Oncology, Maria Sklodowska-Curie Institute-Oncology Center, Warsaw, Poland
3
Department of Otolaryngology, Centre of Postgraduate Medical Education, Kondratowicza 8, 03‑242 Warsaw, Poland
LPS Lipopolysaccharide SNPs Single-nucleotide polymorphisms
Introduction Chronic rhinosinusitis (CRS) is one of the most common health condition affecting nearly 16% of the world’s population (Bhattacharyya et al. 2012), and its prevalence has increased over the last decade. CRS has many known contributing factors inclu
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