Expression of multiple tfb genes in different Halobacterium salinarum strains and interaction of TFB with transcriptiona
- PDF / 664,507 Bytes
- 11 Pages / 595 x 791 pts Page_size
- 36 Downloads / 131 Views
ORIGINAL PAPER
Expression of multiple tfb genes in diVerent Halobacterium salinarum strains and interaction of TFB with transcriptional activator GvpE Anne Bleiholder · Regina Frommherz · Katharina Teufel · Felicitas Pfeifer
Received: 18 May 2011 / Revised: 19 August 2011 / Accepted: 10 September 2011 / Published online: 4 October 2011 © Springer-Verlag 2011
Abstract Halobacterium salinarum NRC-1 contains multiple TBP and TFB proteins required for the recruitment of RNA polymerase for transcription initiation. The presence and the expression of genes encoding TFB were investigated in the two Hbt. salinarum strains NRC-1 and PHH1 and the mutant strain PHH4. The plasmid-encoded tfbC and tfbE genes of NRC-1 were lacking in PHH1 and PHH4. The 5⬘-end of the tfbF transcript was determined and contained a 5⬘-untranslated region of 39 nucleotides able to form a stem-loop structure. The expression of these tfb genes was studied in cultures growing at 15, 37°C and under heat shock conditions. Cold temperatures reduced growth and except for tfbF also the amounts of all tfb transcripts. However, the formation of gas vesicles increased in PHH1 and NRC-1. Heat shock reduced growth of PHH1 and NRC-1, but PHH4 was not aVected. A 100-fold increase in tfbA and tfbB mRNA was observed in PHH1 and PHH4, whereas NRC-1 reduced the amounts of these transcripts and increased the expression of tfbG. All TFB proteins tested were able to interact with the transcription activator GvpE involved in gas vesicle formation that thus is able to recruit TFB to the gvp promoter. Keywords TFB proteins · Genome variation · Alternative tfb genes
Communicated by Erko Stackebrandt. Electronic supplementary material The online version of this article (doi:10.1007/s00203-011-0756-z) contains supplementary material, which is available to authorized users. A. Bleiholder · R. Frommherz · K. Teufel · F. Pfeifer (&) Mikrobiologie und Genetik, Technische Universität Darmstadt, Schnittspahnstr. 10, 64287 Darmstadt, Germany e-mail: [email protected]
Abbreviations TFB Archaeal transcription factor RNAP RNA polymerase qRT-PCR Quantitative reverse transcriptase polymerase chain reaction
Introduction Basal transcription in Archaea requires besides a multi-subunit RNA polymerase (RNAP), the transcription factor TFB and the TATA-box binding protein TBP to recruit RNAP to the promoter. Hyperthermophilic and methanogenic Archaea usually contain a single tbp and single or few tfb genes encoding these general transcription factors (GTFs). Several tfb genes and their diVerential expression were Wrst observed in the haloarchaeon Haloferax volcanii (Thompson et al. 1999). Multiple and divergent GTF genes are present in the genome sequences of the haloarchaeon Halobacterium salinarum NRC-1 and the gas vesicle negative mutant strain R1 (Ng et al. 2000; PfeiVer et al. 2008a, b). The possession of multiple GTFs led to the hypothesis that the formation of diVerent TFB-TBP complexes plays a role in the regulation of transcription initiation and the GTFs constitute a regu
Data Loading...
