Expression of the insulin-like growth factor (IGF) system and steroidogenic enzymes in canine testis tumors
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Expression of the insulin-like growth factor (IGF) system and steroidogenic enzymes in canine testis tumors Monique AJ Peters1,4, Jan A Mol1, Monique E van Wolferen1, Marja A Oosterlaken-Dijksterhuis1, Katja J Teerds2,3 and Frederik J van Sluijs*1 Address: 1Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Universiteit Utrecht, The Netherlands, 2Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Universiteit Utrecht, The Netherlands, 3Department of Animal Sciences, Human and Animal Physiology Group, Wageningen University, The Netherlands and 4Centre for the Quality of Care, University of Nijmegen, The Netherlands Email: Monique AJ Peters - [email protected]; Jan A Mol - [email protected]; Monique E van Wolferen - [email protected]; Marja A Oosterlaken-Dijksterhuis - [email protected]; Katja J Teerds - [email protected]; Frederik J van Sluijs* - [email protected] * Corresponding author
Published: 14 February 2003 Reproductive Biology and Endocrinology 2003, 1:22
Received: 29 January 2003 Accepted: 14 February 2003
This article is available from: http://www.RBEj.com/content/1/1/22 © 2003 Peters et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
Abstract Testis tumors occur frequently in dogs. The main types of tumors are Sertoli cell tumors, seminomas, and Leydig cell tumors. Mixed tumors and bilateral occurrence of tumors may be encountered frequently. To elucidate the possible relationship between the insulin-like growth factor (IGF) system and the development of different types of testis tumors in dogs, the expression of insulin-like growth factor-I and II (IGF-I and IGF-II), their type I receptor (IGF-IR), and their binding proteins (IGFBPs) was examined. In addition the expression of the steroidogenic enzymes p450-aromatase and 5α-reductase type I and type II, and the androgen receptor (AR) was investigated by a semiquantitative reverse-transcriptase PCR (RT-PCR). Both normal testes and testes with tumors were studied. In normal testes a clear expression of IGF-I, IGF-II, IGF-IR, IGFBP2, IGFBP4 and IGFBP5 was found. Expression of IGFBP1 and IGFBP3 was weak. There was also clear expression of the steroidogenic enzymes 5α-reductase, aromatase, and the AR. Quantification of RT-PCR products revealed significantly less expression of IGFBP1, IGF-I, and 5αreductase type I in Sertoli cell tumors and seminomas. Leydig cell tumors and mixed tumors had a significantly higher expression of IGFBP4 and IGF-IR than normal testes. The expression of aromatase was lower in seminomas and in mixed tumors. The expression of AR, IGF-II and IGFBP2, IGFBP3, IGFBP5, and 5α-reductase type II did not differ among the different types of tumors. It was concluded that Sertoli cell tumors and seminomas have a comp
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