Expression profile of serum-related exosomal miRNAs from parathyroid tumor
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ORIGINAL ARTICLE
Expression profile of serum-related exosomal miRNAs from parathyroid tumor Jiacheng Wang1 Qian Wang1 Teng Zhao1 Xing Liu1 Ge Bai1 Yunhui Xin1 Hong Shen1 Bojun Wei ●
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Received: 4 July 2020 / Accepted: 24 October 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Objective The expression pattern of exosomal miRNAs derived from parathyroid tumor is still unknown. In the present work, we aimed to examine the differences on microRNA (miRNA) expression, present in serum exosomes, by comparing parathyroid carcinoma (PC) and parathyroid adenoma (PA). Methods MiRNA expression profile of serum exosomes, derived from 4 PC patients and 4 PA patients, were analyzed by next-generation sequencing technology. The differential expressions of target miRNAs were further verified in both serum exosomes and tissues of PC/PA patients by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Lastly, receiver operating characteristic (ROC) curves were plotted to investigate the efficiency of target exosomal miRNAs in distinguishing PC patients from PA controls. Results Multiple differentially expressed miRNAs of serum exosomes were screened out by sequencing. Based on this screening, hsa-miR-146b-5p (p = 0.0846), hsa-miR-27a-5p (p = 0.0412), hsa-miR-93-5p (p = 0.73), hsa-miR-381-3p (p = 0.1239) and hsa-miR-134-5p (p = 0.0694) were upregulated in the serum exosomes of PC patients. These results were validated by qPCR, where the trend on differential miRNA expression was consistent with the sequencing results. Specifically, the expression of exosomal hsa-miR-27a-5p was able to clearly distinguish PC patients from PA controls, and related analysis indicated that the area under the ROC curve was 0.8594 (p = 0.0157). Conclusions Here we present, for the first time, the miRNA expression profile of serum exosomes derived from PC patients. Based on this result, we presently suggest that the exosomal hsa-miR-27a-5p may serve as a putative tumor marker for preoperative identification of PC and PA subjects. Keywords Parathyroid carcinoma Exosome MicroRNA Expression profile ●
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Introduction Parathyroid carcinoma (PC), a rare endocrine malignancy, is one of the causes of primary hyperparathyroidism (PHPT) and accounts for less than 1% of all PHPT cases [1]. Reportedly, the 5- and 10-year overall survival rates of PC patients are 82.3% and 66%, respectively [2]. An uncontrollable hypercalcemia induced by PC recurrence
Supplementary information The online version of this article (https:// doi.org/10.1007/s12020-020-02535-7) contains supplementary material, which is available to authorized users. * Bojun Wei [email protected] 1
Department of Thyroid and Neck Surgery, Beijing Chao-Yang Hospital, Capital Medical University, 100020 Beijing, China
and metastasis is the leading cause of poor prognosis and mortality of patients afflicted with this malignancy [3]. Therefore, radical resection has been recommended during the initial operati
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