Extracellular ATP affects cell viability, respiratory O 2 uptake, and intracellular ATP production of tobacco cell suspe
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ORIGINAL ARTICLE
Extracellular ATP affects cell viability, respiratory O2 uptake, and intracellular ATP production of tobacco cell suspension culture in response to hydrogen peroxide-induced oxidative stress Qin-zheng Hou 1 & Yu-pei Wang 2 & Bao-qiang Fan 1 & Kun Sun 1 & Jun-yu Liang 1 & Han-qing Feng 1 & Ling-yun Jia 1 Received: 11 October 2018 / Accepted: 31 January 2020 # Institute of Molecular Biology, Slovak Academy of Sciences 2020
Abstract Extracellular adenosine 5′-triphosphate (ATP) is a well-known signalling molecule in plants and plays important roles during plant stress response. In the present work, the treatment of tobacco cell suspension culture with exogenous hydrogen peroxide (H2O2) caused the decreases of respiratory O2 uptake, intracellular ATP production, extracellular ATP level, and reduction of cell viability. Combining this observation with the finding that the oxidative phosphorylation uncoupler or the respiratory inhibitor not only decreased intracellular ATP production but also decreased the extracellular ATP level and cell viability, it is suggested that the decrease of extracellular ATP level and cell viability under H2O2 stress could be a result of the suppressed production of intracellular ATP. Treatment with ATP-degrading enzyme, apyrase, also caused the decreases of cell viability, respiratory O2 uptake, and intracellular ATP production. More importantly, addition of exogenous ATP alleviated the H2O2-induced decreases of cell viability, respiratory O2 uptake, and production of intracellular ATP. These results indicate that extracellular ATP could be an important effector in regulating the cell viability, respiratory O2 uptake, and intracellular ATP production of tobacco cell suspension culture under H2O2 stress. Keywords Extracellular ATP . H2O2 . Cell viability . Respiration . Intracellular ATP
Introduction Adenosine 5′-triphosphate (ATP) is the most important energy currency molecule for all cells. Although the location of ATP is usually viewed in intracellular spaces, much works have revealed that animal, plant, and microbial cells can secrete ATP from the cytosol into extracellular matrix (Parish and Weibel 1980; Boyum and Guidotti 1997; Thomas et al. 2000).
Qin-zheng Hou and Yu-pei Wang are co-first authors Electronic supplementary material The online version of this article (https://doi.org/10.2478/s11756-020-00442-w) contains supplementary material, which is available to authorized users. * Ling-yun Jia [email protected] 1
College of Life Science, Northwest Normal University, Lanzhou 730070, Gansu Province, China
2
Gansu Provincial Maternity and Child-Care Hospital, Lanzhou 730000, Gansu Province, China
In plant cells, intracellular ATP (iATP) can be released into the extracellular matrix by the ATP-binding cassette (ABC) transporters, exocytosis, and plasma membrane-localized nucleotide transporters (Thomas et al. 2000; Kim et al. 2006; Rieder and Neuhaus 2011). Furthermore, extracellular ATP (eATP) is found to play important roles in regulating several physiolog
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