Fabrication of Microfluidic Devices in Thermoplastic Elastomeric Materials for DNA Detection on Thermal Plastic Substrat
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1222-DD05-24
Fabrication of Microfluidic Devices in Thermoplastic Elastomeric Materials for DNA Detection on Thermal Plastic Substrate Kebin Li, Daniel Brassard, François Normandin, Caroline Miville-Godin, Matthias Geissler, Emmanuel Roy and Teodor Veres Industrial Materials Institute, National Research Council, Canada 75, Boul. de Mortagne, Boucherville, Québec, Canada, J4B 6Y4 ABSTRACT Thermoplastic elastomer (TPE) based microfluidic devices integrated with a microfluidic pumping manifold which consists of 4 electromagnetic valves (EMV) were fabricated. The back and forth shuttling flow and its application in the DNA hybridization process were validated on a thermal plastic Zeonor 1060R substrate. The flow rate can be as fast as 23µl/min when the channel width and the channel height are in 100µm, and 25µm, respectively. The DNA hybridization process is detected by using a fluorescence microscopy. Remarkable DNA hybridization is achieved with the continuous flow of the target DNA at a concentration of 10 nM within the first 1 min by using this device. INTRODUCTION Medical diagnostics is an area of the great interest that has recently fuelled an increased interest for the development of disposable rapid diagnostics devices for point-of-care applications. Most of the microfluidic (µ-fluidic) devices are fabricated using either `hard lithography` in semiconductors or glass or `soft lithography` in polymers such as PDMS [1]. It is highly demanded on the fabrication technologies and materials. The rapid and low-cost fabrication, the simple procedure for bonding as well as the stable surface treatments for bio-molecules capture and immobilization are desirable in order to make commercial for the lab-on-chip systems (LOC). On the other hand, the use of DNA microarrays [2,3] based on multiplexed fluorescent measurement has revolutionized the practice of the life sciences research and provided a lot of quantitative information on complex biological systems and many applications such as gene expression analysis or disease diagnostics. The integration of the microarray technologies with µ−fluidic systems can offer the possibility to dramatically reduce the amounts of costly solutions such as DNA probes, proteins or other molecules. It can also provide the prospect for their integration into systems for electrical based detection technologies. Thermoplastic elastomer (TPE) has recently been demonstrated to be biocompatible and surface stable materials that allow us to rapidly fabricate complex µ−fluidic devices at the relatively low cost because of its remarkable advantages in the soft fabrication and µ−fluidic behaviors.[4-6] Herein we report on the fabrication of TPE based µ−fluidic devices by using the hot-embossing technique, the fabricated devices will allow the fast hybridization of DNA probes immobilized on the surface of BioFET arrays. To validate the performance of the devices, some DNA hybridization performance tests were carried out on the thermal plastic substrates. A DNA probe labelled with Cy5 with spot size of
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