Factors affecting simultaneous nitrification and denitrification (SND) in a moving bed sequencing batch reactor (MBSBR)
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RESEARCH PAPER
Factors affecting simultaneous nitrification and denitrification (SND) in a moving bed sequencing batch reactor (MBSBR) system as revealed by microbial community structures Jingyin Wang1 · Hongwei Rong1 · Yongfeng Cao1 · Chaosheng Zhang1 Received: 10 March 2020 / Accepted: 8 May 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The effects of biological factors including dissolved oxygen (DO), pH, carbon/nitrogen (C/N) and hydraulic retention times (HRT) on the performance of simultaneous nitrification and denitrification (SND) in a moving bed sequencing batch reactor (MBSBR) were investigated. A low DO was found to be advantageous to the SND in that nitrification was not inhibited, while pH and C/N ratio were shown to have positive effects on SND, and HRT needed to be controlled in a suitable range. A desirable SND efficiency was obtained at a DO of 2.5 mg L−1, pH of approximately 8.0, C/N ratio of 10 and HRT of 10 h in the MBSBR. High-throughput sequencing analysis showed that different operating conditions impacted microbial communities, resulting in different nitrogen removal mechanisms. Autotrophic and heterotrophic nitrification together contributed to the good nitrification performance, while denitrification was conducted by combined anoxic and aerobic processes. Furthermore, the results of principal component analyses (PCA) and the abundance of the predominant nitrification and denitrification genera both showed that DO and HRT might be regarded as the dominant variable factors influencing community structure analysis during SND, while the linear discriminant analysis (LDA) effect size (LEfSe) algorithm showed differences in abundance among the biofilm microbial communities with different DO. Overall, the results of this study improve our understanding of the bacterial community structure with different operating conditions in MBSBRs. Keywords Moving bed sequencing batch reactor (MBSBR) · Simultaneous nitrification and denitrification (SND) · Microbial community · Biological factor
Introduction The adverse environmental impacts associated with ammonia nitrogen include promotion of eutrophication, toxicity toward aquatic organisms and depletion of dissolved oxygen in receiving water bodies as a result of bacterial oxidation of ammonia to nitrate [1]. Thus, nitrogen removal has become a central issue in modern wastewater treatment plants (WWTP) [2]. The biological elimination of nitrogen Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00449-020-02374-w) contains supplementary material, which is available to authorized users. * Hongwei Rong [email protected] 1
College of Civil Engineering, Guangzhou University, 230 GuangZhou University City Outer Ring Road, Guangzhou 510006, China
in wastewater treatment plants results from the processes of nitrification and de-nitrification, which have been thought to be two separate reactions conducted by different groups of microorganisms in activated sludge. Aerobic autotro
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