Effect of temperature shifts and anammox biomass immobilization on sequencing batch reactor performance and bacterial ge
- PDF / 2,688,843 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 42 Downloads / 238 Views
ORIGINAL PAPER
Effect of temperature shifts and anammox biomass immobilization on sequencing batch reactor performance and bacterial genes abundance A. Banach‑Wiśniewska1 · M. Ćwiertniewicz‑Wojciechowska1 · A. Ziembińska‑Buczyńska1 Received: 15 July 2020 / Revised: 9 September 2020 / Accepted: 23 September 2020 © The Author(s) 2020
Abstract Implementation of anaerobic ammonium oxidation (anammox) below its optimal temperature, known as “cold anammox”, may lead to its common use in wastewater treatment plants, reducing the operational costs of wastewater treatment. Thus, we investigated the effects of immobilization in polyvinyl alcohol–sodium alginate gel beads on anammox performance at temperatures of 30 °C, 23 °C, and 15 °C in laboratory-scale sequencing batch reactors. We determined the relative gene abundance of the nitrogen removal bacterial groups, which are considered as the key functional microbes of nitrogen cycle in activated sludge: denitrifies, ammonia-oxidizing bacteria, nitrite-oxidizing bacteria, and anammox bacteria. Nitrogen removal efficiency was higher for immobilized anammox sludge in comparison with non-immobilized anammox biomass at each investigated temperature. At 30 °C, nitrogen removal efficiency was 83.7 ± 6.46% for immobilized reactor, and 79.4 ± 7.83% for the control reactor, while at 15 °C was remained at the level of 50 ± 2.5% for immobilized reactor, and fluctuated from 13.2 to 45.3% for the control one. During temperature shifts, the process was also more stable in the case of the reactor with immobilized biomass. A statistically significant correlation was found between nitrogen removal efficiency and hydrazine oxidoreductase gene abundance. Keywords Anammox · Immobilization · Polyvinyl alcohol · Relative gene abundance · Sodium alginate
Introduction For decades, biological anaerobic ammonium oxidation (anammox) has been considered as a promising and costeffective process for nitrogen removal from wastewater. Anammox targets wastewater that contains high concentrations of ammonium, such as landfill leachate and digester effluents. The process application could lead to reduction in operational costs of up to 90%. In comparison with traditional denitrification–nitrification methods, the process
Editorial responsibility: Fatih Şen. Date and location of the research carried out throughout the study Research were carried out in Environmental Biotechnology Department, Silesian University of Technology, Poland in 2019. * A. Banach‑Wiśniewska anna.banach‑[email protected] 1
Environmental Biotechnology Department, Faculty of Power and Environmental Engineering, Silesian University of Technology, Akademicka 2, 44‑100 Gliwice, Poland
does not require an external carbon source and allows the reduction in aeration costs. Moreover, anammox application has been linked to reduced greenhouse gas emissions (for instance N2O) (Ma et al. 2016; Cao et al. 2017). However, despite obvious advantages, the application of anammox is limited due to the low growth rate of anammox bacteria, and
Data Loading...
