Faecal microbiota transplant from aged donor mice affects spatial learning and memory via modulating hippocampal synapti
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RESEARCH
Open Access
Faecal microbiota transplant from aged donor mice affects spatial learning and memory via modulating hippocampal synaptic plasticity- and neurotransmissionrelated proteins in young recipients Alfonsina D’Amato1†, Lorenzo Di Cesare Mannelli2†, Elena Lucarini2, Angela L. Man3, Gwenaelle Le Gall4, Jacopo J. V. Branca5, Carla Ghelardini2, Amedeo Amedei5, Eugenio Bertelli6, Mari Regoli6, Alessandra Pacini5, Giulia Luciani5, Pasquale Gallina2,7, Annalisa Altera6, Arjan Narbad8, Massimo Gulisano5, Lesley Hoyles9, David Vauzour4*† and Claudio Nicoletti5*†
Abstract Background: The gut-brain axis and the intestinal microbiota are emerging as key players in health and disease. Shifts in intestinal microbiota composition affect a variety of systems; however, evidence of their direct impact on cognitive functions is still lacking. We tested whether faecal microbiota transplant (FMT) from aged donor mice into young adult recipients altered the hippocampus, an area of the central nervous system (CNS) known to be affected by the ageing process and related functions. (Continued on next page)
* Correspondence: [email protected]; [email protected] † Alfonsina D’Amato and Lorenzo Di Cesare Mannelli contributed equally to the work. † David Vauzour and Claudio Nicoletti share senior authorship. 4 Norwich Medical School, Biomedical Research Centre, University of East Anglia, Norwich NR4 7TJ, UK 5 Dept. of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
D’Amato et al. Microbiome
(2020) 8:140
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Results: Young adult mice were transplanted with the microbiota from either aged or age-matched donor mice. Following transplantation, characterization of the microbiotas and metabolomics profiles along with a battery of cognitive and behavioural tests were performed. Label-free quantitative proteomics
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