Field Evaluation of Sorghum ( Sorghum bicolor ) Lines that Overexpress Two Monolignol-Related Genes that Alter Cell Wall
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Field Evaluation of Sorghum (Sorghum bicolor) Lines that Overexpress Two Monolignol-Related Genes that Alter Cell Wall Composition Hannah M. Tetreault 1,2 & Pat O’Neill 1,3 & John Toy 1 & Tammy Gries 1 & Deanna L. Funnell-Harris 1,3 & Scott E. Sattler 1,2 Received: 6 August 2020 / Accepted: 30 October 2020 # This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020
Abstract Modifying lignin content and composition of cell walls are key targets for bioenergy feedstock improvement for a range of renewable chemical applications. Sorghum (Sorghum bicolor) is a drought-tolerant C4 grass being developed as a dedicated bioenergy feedstock. The monolignol biosynthetic pathway produces the hydroxycinnamic subunits of lignin, a major target to increase the energy content of sorghum biomass for thermal bioenergy conversion processes. SbMyb60 is a transcription factor linked to the activation of the monolignol biosynthetic pathway and caffeoyl CoA O-methyltransferase (SbCCoAOMT) is a Sadenosyl methionine (SAM)–dependent O-methyltransferase responsible for the methylation of caffeoyl-CoA to generate feruloyl-CoA in this pathway. Overexpression of each gene resulted in increased energy content of biomass and increased levels of several aromatic compounds in previous greenhouse experiments. To assess how SbMyb60 and SbCCoAOMT overexpression lines performed under field conditions, these lines were planted in field trials over two growing seasons, 2017 and 2018, and the effects on lignin composition and content as well as agronomic traits were assessed. Field-grown plants of SbMyb60 and SbCCoAOMT overexpression lines had increased total energy and phenolic compounds. Furthermore, two SbMyb60 transgenic events, SbMyb-10a and SbMyb-15a, with decreased stover yield and seed set were combined with three sorghum lines, N32, N4692, and Tx3118, to investigate whether yield reduction could be rescued. Hybrids improved yield of SbMyb60-15a; however, hybrids were unable to improve performance for SbMyb60-10a. Overall, these results demonstrated that field-grown sorghum overexpressing SbMyb60 or SbCCoAOMT can have biomass with increased phenolic compounds and total energy. Keywords Sorghum (Sorghum bicolor) . Monolignol biosynthetic pathway . Bioenergy feedstock . SbMyb60 and SbCCoAOMT
Introduction Sorghum (Sorghum bicolor), a C4 grass with its origin in Africa, is receiving substantial attention as a bioenergy crop, but it is also a versatile cereal crop grown in marginal environments around the world [28]. The genetic diversity and wide adaptation of sorghum varieties provide breeding programs with a wide array of unique traits to use in breeding * Scott E. Sattler [email protected] 1
Wheat, Sorghum and Forage Research Unit, USDA-ARS, Lincoln, NE 68583, USA
2
Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68583, USA
3
Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE 68583, USA
efforts [15]. Increasing b
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