Fine mapping of qSdr9 , a novel locus for seed dormancy (SD) in weedy rice, and development of NILs with a strong SD all
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Fine mapping of qSdr9, a novel locus for seed dormancy (SD) in weedy rice, and development of NILs with a strong SD allele Thanhliem Nguyen & Kai Fu & Changling Mou & Jiangfeng Yu & Xingjie Zhu & Yunshuai Huang & Chunlei Zhou & Qixian Hao & Fulin Zhang & Weihan Song & Ping Wang & Yaping Chen & Tengfei Ma & Yunlu Tian & Shijia Liu & Ling Jiang & Jianmin Wan
Received: 21 April 2020 / Accepted: 2 July 2020 # Springer Nature B.V. 2020
Abstract Lack of seed dormancy, a major cause of preharvest sprouting in rice and other cereal crops, causes significant reductions in grain yield and quality. Weedy rice is often strongly dormant; therefore, quantitative trait loci (QTLs) related to seed dormancy in weedy rice “Ludao” could be useful for improving pre-harvest sprouting resistance in the important japonica variety “Ninggeng 4.” To identify SD QTLs in variety Ludao, we constructed an advanced backcross population (BC6F2) of Ninggeng 4 with Ludao as the donor parent by markerassisted backcrossing followed by SD phenotyping. The consistently detected QTL qSdr9 on chromosome 9L explained 18.09% of the phenotypic variance, and the Ludao allele decreased germination percentage by 10.78%. qSdr9 was fine-mapped to a 586.60 kb region between markers QNU9–10 and RM5777. Based on the closest linked marker for locus qSdr9, we developed near-isogenic lines with the qSdr9 allele from Ludao in Ninggeng 4
background. The qSdr9 near-isogenic lines with strong SD will be useful as parents in breeding for pre-harvest sprouting resistance. Keywords Near-isogenic line . Pre-harvest sprouting . QTL . Oryza sativa
Introduction Germination is the physiological process in seeds that begins with water uptake by a harvest-ripe seed and terminates with protrusion of the embryonic axis, usually the radicle, from the covering tissues. Seed dormancy (SD) is defined as the temporary failure of a viable seed to complete germination under favorable conditions (Bewley 1997). Pre-harvest sprouting (PHS) involves germination of seeds on plants before harvesting. Crops are pre-
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11032-020-01148-2) contains supplementary material, which is available to authorized users. T. Nguyen : K. Fu : C. Mou : J. Yu : X. Zhu : Y. Huang : C. Zhou : Q. Hao : F. Zhang : W. Song : P. Wang : Y. Chen : T. Ma : Y. Tian : S. Liu : L. Jiang (*) : J. Wan (*) State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China e-mail: [email protected] e-mail: [email protected]
T. Nguyen Faculty of Natural Sciences, Quynhon University, Quynhon, Binhdinh 590000, Vietnam J. Wan National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
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disposed to PHS when the un-harvested seeds encounter prolonged rainfall or periods of high humidity. The extreme condition known
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