Seed Dormancy Methods and Protocols
Understanding seed-related processes is of major social, environmental, and economic concern. The viability and vigor of seeds are the very basis for sustainable agriculture and forestry, and comprehending the molecular and cellular events underlying thes
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
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Seed Dormancy Methods and Protocols
Edited by
Allison R. Kermode Department of Biological Sciences, Simon Fraser University, Burnaby, BC, Canada
Editor Allison R. Kermode Department of Biological Sciences Simon Fraser University Burnaby, BC Canada [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-230-4 e-ISBN 978-1-61779-231-1 DOI 10.1007/978-1-61779-231-1 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011933570 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface This book covers analytical methods and approaches, which have led to significant advances in our understanding of seed dormancy and germination and have the potential to lead to new avenues of research. The methods detailed in this book are of value for both applied and fundamental seed research, and range from whole seed batch treatments to the analysis of tissues or even single seeds at the DNA, protein, and metabolite levels. Methods are described in detail and critical steps explained so that several of the chapters of this book can serve both as a protocol and a guideline for their adaptation and optimization to new applications or species. The viability and vigor of seeds are the very basis for sustainable agriculture and forestry, and understanding the molecular and cellular events underlying these processes will become increasingly important to many economical sectors and for species that provide the world’s food supply. Seeds are also the focus of environmental conservation efforts aimed at preserving the floral biodiversity of vanishing habitats. Understanding seedrelated processes is thus of major social, environmental, and economical concern. The main foci of seed-related research are the regulation of lifecycle transitions, such as the dormancy-to-germination and germination-to-growth transitions; seed viability, quality, and vigor are central as well, and are influenced by events that take place during seed development. Some orthodo
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