First isolation and genetic characterization of pseudocowpox virus from cattle in Japan
- PDF / 696,755 Bytes
- 5 Pages / 595.276 x 790.866 pts Page_size
- 94 Downloads / 211 Views
SHORT REPORT
Open Access
First isolation and genetic characterization of pseudocowpox virus from cattle in Japan Akifumi Ohtani1, Akihiro Yokoyama1, Hisato Narushige1 and Yasuo Inoshima2,3,4*
Abstract Background: Pseudocowpox virus (PCPV) infects cattle worldwide with zoonotic potential but has not been isolated in Japan. Thus, the epidemiological status of PCPV infection in cattle is undetermined. Results: In May 2016, a cattle in a farm in Yamaguchi Prefecture showed white vesicles and hyperemia in the mucosa under the tongue surface, but not on the teats and coronary cushions. A parapoxvirus was isolated from the oral lesion swab and was genetically characterized based on the full-length sequence of B2L gene encoding viral envelope. Phylogenetic analysis showed that the isolated virus was classified into PCPV. Conclusion: This case indicates its potential spread in Japan. This is the first report of isolation of PCPV in Japan. Keywords: Pseudocowpox virus, Cattle, Oral lesions, Isolation, B2L gene
Background Pseudocowpox virus (PCPV) is a member of the genus Parapoxvirus in the family Poxviridae, which includes bovine papular stomatitis virus (BPSV) and orf virus (ORFV) [1]. Parapoxviruses are commonly known as causative agents of dermal diseases in ruminants worldwide, leading to papular stomatitis and contagious pustular dermatitis, especially in the regions of the lips, nostrils, oral mucosa, and teats. The importance of PCPV is increasingly recognized, primarily because of economic losses to farmers in connection with disease outbreaks and because of their zoonotic potential [2]. In Japan, although serological surveys have revealed that seroprevalence of parapoxvirus is very high in cattle and sheep [3, 4] and multiple BPSVs have been isolated [5], no PCPV has yet been isolated; thus, the epidemiological status of PCPV infection in cattle is undetermined. We here report the first case of the isolation of PCPV in Japan. We determined the full-length sequence of the B2L gene encoding viral envelope of this isolate, and evaluated its phylogenetic relation to known members of this virus group. * Correspondence: [email protected] 2 Laboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan 3 The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan Full list of author information is available at the end of the article
Methods Clinical and epidemiological investigations
In May 2016, a breeding cow (Japanese Black, female, 13month old) in a farm in Yamaguchi Prefecture, in the western part of Japan showed anorexia, mild fever, frothy salivation, and hyperemia in the mucosa under the tongue surface. No lesions were observed on the teats or coronary cushions. A few days after the onset of clinical signs, the cattle showed white vesicles on the tongue surface (Fig. 1). These signs were convalescent in about 1 week. No other cattle in the herd showed clinical signs. Sa
Data Loading...
