First report of meticillin-resistant Staphylococcus aureus harboring mecC gene in milk samples from cows with mastitis i
- PDF / 406,612 Bytes
- 5 Pages / 595.276 x 790.866 pts Page_size
- 41 Downloads / 165 Views
VETERINARY MICROBIOLOGY - SHORT COMMUNICATION
First report of meticillin-resistant Staphylococcus aureus harboring mecC gene in milk samples from cows with mastitis in southeastern Brazil Maria de Fatima N. F. Alves 1,2,3 & Bruno Penna 4,5 Helena Carla Castro 3,6 & Fabio Aguiar-Alves 1,5,6
&
Renata F. A. Pereira 1,3 & Reinaldo B Geraldo 2,3 & Evelize Folly 2,3 &
Received: 1 August 2020 / Accepted: 15 October 2020 # Sociedade Brasileira de Microbiologia 2020
Abstract MRSA infection and colonization have been reported in both companion and food-chain animals, highlighting MRSA as an important veterinary and zoonotic pathogen. Another mec allele, the mecC gene, also confers beta-lactam resistance in Staphylococcus aureus and shows 69% nucleotide identity to mecA. The main aim of this study was to investigate the genotypic and clonal profile of methicillin-resistant S. aureus (MRSA) from cows with mastitis in dairy herds. Thirty-five samples suggestive of bovine subclinical mastitis were evaluated, and S. aureus were detected in all of them using both phenotypic and molecular approaches. According to the multilocus sequence typing (MLST), the S. aureus isolates were assigned in five different STs, 21 (60%) showed ST 742, 6 (17%) ST97, 4 (11%) ST1, 2 (6%) ST30, and 2 (6%) ST126. The presence of mecA was not observed in any of these isolates whereas mecC was detected in nine of them (9/35; 26%). The Panton-Valentine leukocidin (PVL) genes were detected in a total of 4 isolates. Among the 35 isolates analyzed, 26 showed resistance to penicillin. Changes in the S. aureus epidemiology due to the detection of MRSA in milk samples from cows presenting with bovine subclinical mastitis may have consequences for public health in Brazil, challenging the empirical therapy and animal management, with potential medical and social outcomes. To the best of our knowledge, this is the first report describing mecC MRSA in Southeastern Brazil. Keywords Staphylococcus aureus . MRSA . mecC . Bovine mastitis
Methicillin-resistant S. aureus (MRSA) are still significant human and animal pathogens, causing serious and lethal infections, with inestimable public health consequences and major economic impacts [1]. MRSA strains normally contain mecA, a gene that encodes for the penicillin-binding protein 2a (PBP2a). This modified enzyme induces resistance to
virtually all β-lactam antibiotics, making MRSA a global public health concern [2]. MRSA cases have emerged in production animals in the last years [3]. These bacteria can be transferred to man by direct contact or as food contaminants [4]. A mecA homolog in S. aureus, the mecC, which confers resistance to beta-
Responsible Editor: Agnes M.S. Figueiredo * Bruno Penna [email protected] * Evelize Folly [email protected]; [email protected] 1
2
Laboratório de Epidemiologia Molecular e Biotecnologia Laboratório Universitário Rodolfo Albino, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil Laboratório de Estudos em Pragas e Parasitos - Instituto de Biologia, Universida
Data Loading...
