First-time comparative investigation on Araucaria columnaris (G. Forst.) Hook. leaves: extraction techniques, phytochemi
- PDF / 1,866,130 Bytes
- 19 Pages / 595.276 x 790.866 pts Page_size
- 2 Downloads / 187 Views
Medicinal Chemistry Research https://doi.org/10.1007/s00044-020-02650-0
ORIGINAL RESEARCH
First-time comparative investigation on Araucaria columnaris (G. Forst.) Hook. leaves: extraction techniques, phytochemicals, medicinal activities, and DFT study Poonam K. Patial1 Damanjit S. Cannoo ●
1
1234567890();,:
1234567890();,:
Received: 26 April 2020 / Accepted: 8 October 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract The present study was aimed to explore the phytochemical composition, antioxidant potential, and density function theory (DFT) study of Araucaria columnaris (G. Forst.) Hook. leaves. Different extraction techniques like Soxhlet, ultrasound, and maceration were performed using solvents—hexane, chloroform, and methanol. Among the three solvents, the best antioxidant values were observed in methanolic extracts followed by chloroform and hexane. The results of RP-HPLC-DAD showed the 11 polyphenolic compounds in methanolic extracts. The observed order of yield, polyphenolic content and antioxidant potential in three techniques was SOX > ULT > MCE. For the first time, 48 bioactive compounds were identified in methanol, chloroform, and hexane extracts using GC–MS technique. DFT has been carried out on major phytochemicals of methanol extract at B3LYP/6–31G (d) basis set (Gaussian 09 program). The obtained results concluded that the A. columnaris (G. Forst.) Hook. leaves contained the distinct phytochemicals having ethnopharmacological employments and significant antioxidant potential. Graphical Abstract
Keywords Araucaria columnaris (G. Forst.) Hook Phytochemical Antioxidant RP-HPLC-DAD and GC–MS DFT ●
●
●
●
Introduction
Supplementary information The online version of this article (https:// doi.org/10.1007/s00044-020-02650-0) contains supplementary material, which is available to authorized users. * Damanjit S. Cannoo [email protected] 1
Department of Chemistry, Sant Longowal Institute of Engineering & Technology, SLIET (Govt. of India, Deemed University), Longowal, Sangrur, Punjab 148106, India
The multifunctional application of natural products is due to their antioxidant mechanism where they can inhibit the redox processes of reactive oxygen species (ROS) [1]. ROS are the by-products produced during the mitochondrial redox reactions. These reactive species (like O2−, ·OH, 1O2, and H2O2) can interact with other biological molecules to stable themselves which leads to harm to the DNA, proteins, and lipids [1, 2]. Such conditions cause various diseases including cell-damaging or aging, and cancer cell growth. The antioxidant mechanism of natural products is
Medicinal Chemistry Research
because of their phenolic/polyphenolic compounds. The phenolic/polyphenolic compounds are helpful to cure the disease like cancer, HIV, cardiovascular, inflammation, etc. through the antioxidant mechanism [3]. India is well known for its medicinal plants where more than 20,000 plants are found. These days, the consumption of medicinal plants has been consistently increa
Data Loading...
