Florescence Imaging Lung Cancer with a Small Molecule MHI-148
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ORIGINAL ARTICLE
Florescence Imaging Lung Cancer with a Small Molecule MHI-148 Xiaotian Xia 1,2 & Yongkang Gai 1,2 & Hongyan Feng 1,2 & Chunxia Qin 1,2 & Dongfeng Pan 3 & Yiling Song 1,2 & Yongxue Zhang 1,2 & Xiaoli Lan 1,2 Received: 29 January 2020 / Accepted: 4 August 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract MHI-148 is a type of heptamethine cyanine dye that can cross the cytoplasmic membrane of lung cancer cells. Here we tested the cytotoxic, in vivo imaging of MHI-148 in lung-cancer nude mice model. Ex vivo imaging was also been measured by testing the major tissue fluorescence intensity. And, the small molecular compound MHI-148 had low cytotoxicity which could be visualized at 1 h post-injection in tumor. From ex vivo fluorescence imaging, the tumor showed the highest uptake of MHI-148 among all the selected organs expect for the time point of 2 h. MHI-148 could be used for effective imaging in lung cancer tissue with good stability and specificity, which suggested that MHI-148 could be an effective tumor clinical imaging agent. Keywords Near infrared fluorescence imaging . MHI-148 . Lung cancer . Organic anion transporting polypeptide
Introduction The incidence and mortality of lung cancer are ranked first among all cancers and will continue to increase in China over the next 20 years [1, 2]. Early diagnosis of lung cancer will effectively reduce its mortality rate. Molecular imaging approaches are effective tools for cancer detection and prognosis, allowing various approaches for visualization of tumors [3]. Magnetic resonance imaging (MRI), positron emission tomography (PET), single photon emission computed tomography (SPECT), ultrasound and optical imaging are being used for tumor detection and visualization [4, 5]. However, the identification of new optimal pharmacological tumortargeted probes is still needed [6, 7]. Light production in fluorescence imaging is based on the excitation of a fluorescent molecule with visible light, resulting in emitted light of lower energy and longer
* Xiaoli Lan [email protected]; [email protected] 1
Department of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1277 Jiefang Ave, Wuhan 430022, Hubei Province, China
2
Hubei Province Key Laboratory of Molecular Imaging, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
3
Department of Radiology, University of Virginia, Charlottesville, VA 22908, USA
wavelength that can be used for imaging purposes [8]. The development of near infrared (NIR) fluorophores and nanomaterials has facilitated the translation of fluorescence imaging from the microscopic (epifluorescence, confocal and multifocal microscopy) to macroscopic imaging (fluorescence molecular tomography, fluorescence reflectance imaging) [9, 10]. Other biological components can translate into an optical imaging window from approximately 600 to 1000 nm in which the absorption coefficient of tissue is at
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