Fluorescence Polarization Immunoassay for Highly Efficient Detection of Imidaclothiz in Agricultural Samples
- PDF / 672,177 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 30 Downloads / 207 Views
Fluorescence Polarization Immunoassay for Highly Efficient Detection of Imidaclothiz in Agricultural Samples Ming Ma 1,2 & Mo Chen 1,2 & Lu Feng 1,2 & Hongjie You 1,2 & Rui Yang 1,2 & Anna Boroduleva 3 & Xiude Hua 1,2 & Sergei A. Eremin 3 & Minghua Wang 1,2
Received: 5 November 2015 / Accepted: 1 February 2016 # Springer Science+Business Media New York 2016
Abstract A homogeneous fluorescence polarization immunoassay (FPIA) based on a monoclonal antibody for the detection of imidaclothiz was developed. Two fluoresceinlabeled imidaclothiz tracers containing two different bridge lengths were synthesized and purified. Under optimal conditions, the 4-aminofluorescein-labeled imidaclothiz conjugate (AMF-labeled imidaclothiz), which contains a shorter bridge length, showed a higher sensitivity in the FPIA for detecting imidaclothiz, and the full analysis was achieved in less than 11 min. The IC50 and limit of detection (LOD, IC10) were 87.94 ± 10.18 and 0.57 ± 0.16 μg/L, respectively. The spiked recoveries were 83 to 117 % measured in tomato, pear, rice, apple, cucumber, cabbage, and paddy water, with RSDs of 5 to 12 %. Furthermore, the results of FPIA for the authentic samples correlated well with those acquired by HPLC. Overall, the developed FPIA provided a simple, rapid, sensitive, and accurate method that was used for the quantitative detection of imidaclothiz in agricultural samples.
Electronic supplementary material The online version of this article (doi:10.1007/s12161-016-0434-5) contains supplementary material, which is available to authorized users. * Minghua Wang [email protected]
1
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
2
State and Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China
3
Faculty of Chemistry, M. V. Lomonosov Moscow State University, Moscow 119991, Russia
Keywords Imidaclothiz . Fluorescence polarization immunoassay . Monoclonal antibodies . Fluorescein-labeled imidaclothiz tracers
Introduction Imidaclothiz, a member of the neonicotinoid insecticides with greater systemic activity, has been widely used for controlling whiteflies, thrips, leafhoppers, plant hoppers, and other various harmful pest species in China (Zhang 2005; Lan 2006; Xu et al. 2007). The action site locates insect nicotinergic acetylcholine receptors (Feng et al. 2008). It was developed and first commercialized by Nantong Jiangshan Agrochemical and Chemicals Co., Ltd. (China) (Dai 2005). In recent years, with the wide use of new neonicotinoid pesticides, the negative effects have become increasingly prominent. Neonicotinoid pesticides could affect honey bees’ ability to collect nectar and could indirectly harm the pollinators (Henry et al. 2012). Therefore, monitoring the residual imidaclothiz in environmental and agricultural samples is significant. The use of immunoassays as a rapid detection technology has been widely used for the detection of small molecules with many advantages, such as simplicity, low consumpt
Data Loading...
