Fluorescence Spectroscopy in Biology Advanced Methods and their Appl

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Fluorescence Spectroscopy in Biology Advanced Methods and their Applications to Membranes, Proteins, DNA, and Cells

Volume Editors: M. Hof · R. Hutterer · V. Fidler

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About this series: Fluorescence spectroscopy, fluorescence imaging and fluorescent probes are indispensible tools in numerous fields of modern medicine and science, including molecular biology, biophysics, biochemistry, clinical diagnosis and analytical and environmental chemistry. Applications stretch from spectroscopy and sensor technology to microscopy and imaging, to single molecule detection, to the development of novel fluorescent probes, and to proteomics and genomics. The Springer Series on Fluorescence aims at publishing stateof-the-art articles that can serve as invaluable tools for both practitioners and researchers being active in this highly interdisciplinary field.The carefully edited collection of papers in each volume will give continuous inspiration for new research and will point to exciting new trends.

Library of Congress Control Number: 2004114543 ISSN 1617-1306 ISBN 3-540-22338-X Springer Berlin Heidelberg New York This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilm or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the provisions of the German Copyright Law of September 9, 1965, in its current version, and permission for use must always be obtained from Springer-Verlag. Violations are liable for prosecution under the German Copyright Law. Springer is a part of Springer Science+Business Media springeronline.com © Springer-Verlag Berlin Heidelberg 2005 Printed in Germany The use of general descriptive names, registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Cover-design: design and production GmbH Dataconversion: Fotosatz-Service Köhler GmbH, Würzburg Printed on acid-free paper 52/3020xv – 5 4 3 2 1 0

Series Editor Professor Dr. Otto S. Wolfbeis University of Regensburg Institute of Analytical Chemistry, Chemo- and Biosensors Universitätsstraße 31 93040 Regensburg Germany e-mail: [email protected]

Volume Editors Professor M. Hof J. Heyrovský Institute of Physical Chemistry Academy of Sciences of the Czech Republic and Centre for Complex Molecular Systems and Biomolecules Dolejškova 3 18223 Prague 8 Czech Republic e-mail: [email protected] Professor R. Hutterer Institute of Analytical Chemistry, Chemo- and Biosensors Universitätsstraße 31 93040 Regensburg Germany e-mail: [email protected] Professor V. Fidler Faculty of Nuclear Sciences and Physical Engineering Czech Technical University in Prague Břehová 7 11519 Praha 1 Czech Republic e-mail: fidl