Topics in Fluorescence Spectroscopy

Fluorescence spectroscopy and its applications to the physical and life sciences have evolved rapidly during the past decade. The increased interest in fluorescence appears to be due to advances in time resolution, methods of data analysis and improved in

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Topics in Fluorescence Spectroscopy Edited by JOSEPH R. LAKOWICZ

Volume 1: Techniques Volume 2: Principles Volume 3: Biochemical Applications

Topics in Fluorescence Spectroscopy Volume 3 Biochemical Applications

Edited by

JOSEPH R. LAKOWICZ Center for Fluorescence Spectroscopy Department of Biological Chemistry University of Maryland School of Medicine Baltimore, Maryland

KLUWER ACADEMIC PUBLISHERS NEW YORK, BOSTON, DORDRECHT, LONDON, MOSCOW

eBook ISBN: Print ISBN:

0-306-47059-4 0-306-43954-9

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Contributors

S. Arnold • Microparticle Photophysics Laboratory (MP 3 L), Department of Physics, Polytechnic University, Brooklyn, New York 11201 Daniel Axelrod • Department of Physics and Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109

A. P. Demchenko • A. V. Palladin Institute of Biochemistry of the Academy of Sciences, Kiev 252030, Ukraine L. M. Folan • Microprarticle Photophysics Laboratory (MP 3 L), Department of Physics, Polytechnic University, Brooklyn, New York 11201 Bryant S. Fujimoto • Department of Chemistry, University of Washington, Seattle, Washington 98195 Robert M. Fulbright • Department of Physics and Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109 Edward H. Hellen • Department of Physics and Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109

William R. Laws • Department of Biochemistry, Mount Sinai School of Medicine, New York, New York 10029 Thomas M. Li • Development Department, Syva, Palo Alto, California 94304 Richard F. Parrish • Development Department, Syva, Palo Alto, California 94304

J. B. Alexander Ross • Department of Biochemistry, Mount Sinai School of Medicine, New York, New York 10029

Kenneth W. Rousslang • Department of Chemistry, University of Puget Sound, Tacoma, Washington 98416 J. Michael Schurr • Department of Chemistry, University of Washington, Seattle, Washington 98195 v

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Contributors

Lu Song • Department of Chemistry, University of Washington, Seattle, Washington 98195 Christopher D. Stubbs • Department of Pathology and Cell Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107 Jane M. Vanderkooi • Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104

Brian Wesley Williams • Department of Chemistry, Bucknell University, Lewisburg, Pennsylvania 17837 Pengguang Wu • Department of Chemistry, University of Washington, Seattle, Washington 98195

Herman R. Wyssbrod • Department of Chemistry, University of Louisville, Louisville, Kentucky 40292

Preface

Fluorescence s