Freezing Assisted Protein Delivery by Using Polymeric Cryoprotectant
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Freezing Assisted Protein Delivery by Using Polymeric Cryoprotectant Sana AHMED1,2 and Kazuaki MATSUMURA1 1
School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292 Japan 2
M.Tech (Chemical Synthesis and Process Technologies), Department of Chemistry, University of Delhi, Delhi-110007, India ABSTRACT A number of drug carrier systems such as liposomes, polymeric-nanoparticles, microparticles, polymeric micelles have been investigated for intracellular delivery. Among these liposomes are the potential drug vehicles for efficient cytosolic delivery. They have an adhesive property for cell membrane to encapsulate the drug or protein effectively and showing the enhanced absorption rate. One of the problems could be the difficulty of incorporation of the drug or protein into cell. Therefore many studies of the drug carriers have been developed to enhance the intracellular delivery of materials. Here we propose the novel method to improve the intracellular uptaking by using freeze concentration. Solutes are excluded from ice crystallization and concentrated in the remaining solution during freezing by freezing concentration. We have reported that polymeric cryoprotectant which is carboxylated poly-L-lysine was adsorbed on to the cell membrane during freezing and caused effective freeze concentration. In this study we investigated that delivery of protein effectively taking place by liposome as a carrier agent. It was successfully delivered protein to L929 cells via freeze concentration using polymeric cryoprotectant as a novel drug delivery. INTRODUCTION Over the past few decades drug delivery vehicles has received a great attention and growing interest in the field of nanomedicine and therapeutic technology. Effective intracellular drug delivery is an important for beneficial agents which have specific targets inside a cell. Liposomes have been widely investigated as a drug carrier for delivery of therapeutic agents to specific sites in the body [1-3]. Liposomal vesicles have drawn attention of researchers as a potential carriers of various bioactive molecules that could be used for therapeutic applications. The major advances in liposome technology has the ability to produce precise liposomes, broad variety of lipids with different physical and chemical properties, high drug trapping efficiency, average less than 100 nm in diameter. Recent studies reveal that cells were cryopreserved successfully by using carboxylated poly-Llysine as a polymeric cryoprotectant [4-5]. We are attempted to use the concept of cryopreservation is to combine in protein delivery using liposome as a vehicles. The aim of this study is to focuse on protein delivery using polymeric cryoprotectant and induced by freeze
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concentration mechanism. During freezing, ice can form in the extracellular space leading to increasing concentrations of electrolytes in the remaining extracellular solution which is called freeze concentration. Therefore, solutes in the extracellular solution cou
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