Functional analysis of rubber tree receptor-like cytoplasmic kinase HbBIK1 in plant root development and immune response
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ORIGINAL ARTICLE
Functional analysis of rubber tree receptor-like cytoplasmic kinase HbBIK1 in plant root development and immune response Shengmin Zhang 1 & Suxia Guo 2 & Liping Feng 1 & Qiannan Wang 1 & Bang An 1 & Hongli Luo 1
&
Chaozu He 1
Received: 21 July 2020 / Revised: 18 October 2020 / Accepted: 16 November 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Nature rubber from rubber tree is one of the most important raw materials for industry. However, rubber trees have been seriously affected by fungal diseases, resulting in large yield loss of nature rubber. Therefore, it is important to identify the disease resistance–related genes for genetically engineered breeding of disease resistance in rubber tree. In this study, the homologous gene of Arabidopsis thaliana receptor-like kinase AtBIK1, HbBIK1, was selected from rubber tree. The full length of HbBIK1 gene was 1260 bp of 419 aa with an intracellular kinase motif. HbBIK1 expression was induced by Oidium heveae Steinmann infection, indicating that HbBIK1 might be involved in response to O. heveae Steinmann infection. The expression level of HbBIK1 was also induced by jasmonic acid (JA) treatment, but not by salicylic acid (SA) and ethylene (ET) treatments. In addition, HbBIK1 was specifically localized on plasma membrane of rubber tree mesophyll protoplasts. Transgenic HbBIK1 lines using AtBIK1 native promoter could rescue not only root length but also disease sensitivity to Alternaria brassicicola in A. thaliana bik1 mutant, indicating the functional similarity between AtBIK1 and HbBIK1. These results showed that HbBIK1 might involve in plant disease resistance and could be used as a candidate disease-related gene for defense genetic breeding in rubber tree. Keywords Hevea brasiliensis . Receptor-like protein kinase . Disease resistance . HbBIK1
Introduction Plants have evolved a sophisticated innate immune system to detect and defend various pathogen attacks. Plant innate immunity mainly comprises two perception systems. One is the perception of conserved pathogen/microbe-associated molecular patterns, PAMPs/MAMPs, by host cell surface immune receptors, also called pattern-recognition receptors (PRRs), which triggers PAMP-triggered immunity (PTI) (Jones and
Dangl 2006). PTI activation was mediated by activated PRR complexes which were composed of receptor-like kinases (RLKs) and receptor-like proteins (RLPs) (Tang et al. 2017; Mithoe and Menke 2018). Another perception involves the recognition of pathogen virulence factors called effectors by intracellular immune receptors, which induces effectortriggered immunity (ETI) (Dodds and Rathjen 2010). Unlike ETI, PTI is a basal immune response against a broad spectrum of pathogens (Mazzotta and Kemmerling 2011). So far, the
Communicated by C. Dardick * Hongli Luo [email protected]
Qiannan Wang [email protected]
* Chaozu He [email protected] Shengmin Zhang [email protected]
Bang An [email protected] 1
Hainan Key Laboratory for Sustainable Utilization of
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