Characterization of Arabidopsis thaliana Root-Related Mutants Reveals ABA Regulation of Plant Development and Drought Re
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Characterization of Arabidopsis thaliana Root‑Related Mutants Reveals ABA Regulation of Plant Development and Drought Resistance Huan Dong1,2 · Xiaonan Ma1 · Pei Zhang3 · Huan Wang4 · Xiaoli Li1 · Jiaxing Liu1 · Ling Bai1 · Chun‑peng Song1 Received: 2 May 2019 / Accepted: 18 January 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract The plant hormone abscisic acid (ABA) regulates many processes of plant growth and development. ABA receptors have been identified in studies of the ABA response of stomatal movement, but the underlying mechanisms of ABA-regulated root growth and development are unclear. To address these questions, we screened for Arabidopsis thaliana mutants based on the sensitivity of root growth to exogenous ABA, using ethyl methanesulfonate-mutagenized (EMS) and T-DNA insertion mutant libraries. We identified 11 mutants, termed roa1–roa11, with Root growth Overly sensitive to ABA (ROA) phenotypes, and cloned two of the ROA genes, one by thermal asymmetric interlaced PCR technology (ROA3) and the other by map-based cloning (ROA9). The roa mutants were also found to have defects in other major ABA responses, including ABA-mediated seed germination and drought resistance. The roa mutants provide crucial genetic material for further studies of ABA signaling and regulatory mechanisms in root growth and development. Keywords Root growth · ABA responses · Seed germination · Drought resistance
Introduction Plants display remarkable developmental plasticity, including in their roots (Péret et al. 2009a, b; De Smet et al. 2012). Through differential growth, roots can change their shape and distribution within the soil and adapt to changes in their environment (Fitter 1991; Kramer and Boyer 1995; LópezBucio et al. 2003). Structurally, the root system is simple and grows solely through reiteration and elongation of root Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00344-020-10076-6) contains supplementary material, which is available to authorized users. * Ling Bai [email protected] 1
Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Biology, Institute of Biomedical Informatics, Henan University, 85 Minglun Street, Kaifeng 475001, Henan, China
2
College of Life Sciences, Henan Agricultural University, Zhengzhou 475000, Henan, China
3
Experimental Center of Tropical Forestry, Chinese Academy of Forestry, Pingxiang 532600, Guangxi, China
4
School of Marine Science, Ningbo University, Ningbo 315211, Zhejiang, China
organs. This simplicity, coupled with the roots’ sensitivity to signals from the soil environment, makes roots an ideal model for research into the determinants of plant architecture and developmental plasticity (Malamy 2005). Moreover, the structure of a plant’s root system has a direct impact on the plant’s use of water and nutrients, thereby controlling signal transduction from root to shoot and determining the development and survival of the whole plant.
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