Functional analysis of a regulator of G-protein signaling CgRGS1 in the rubber tree anthracnose fungus Colletotrichum gl
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ORIGINAL PAPER
Functional analysis of a regulator of G-protein signaling CgRGS1 in the rubber tree anthracnose fungus Colletotrichum gloeosporioides Zhi‑Qiang Liu1 · Man‑Li Wu1 · Zhi‑Jian Ke1 · Wen‑Bo Liu1 · Xiao‑Yu Li1 Received: 24 June 2017 / Revised: 12 November 2017 / Accepted: 14 November 2017 © Springer-Verlag GmbH Germany, part of Springer Nature 2017
Abstract Colletotrichum gloeosporioides is the causal agent of rubber anthracnose, which is also one of the important biological factors threatening the development of natural rubber industry in the world. Regulators of G-protein signaling (RGS) are key negative regulators of G-proteins, which play important roles in growth, development and pathogenic processes of plant pathogens. In this study, a RGS gene CgRGS1 was functionally characterized in C. gloeosporioides. Compared to the wild type, the CgRGS1 deletion mutant had slow vegetative growth, reduced conidia with multi-end germination, low appressorium formation rate, high resistance to oxidative stress and SDS. Moreover, the mutant was sensitive to osmotic pressure and showed decreased virulence. In conclusion, CgRGS1 is involved in regulation of vegetative growth, conidiation, germination, appressorium formation, oxidative stress, osmotic pressure response and pathogenicity in C. gloeosporioides. Keywords Rubber anthracnose · Colletotrichum gloeosporioides · Regulators of G-protein signaling · Biological function
Introduction Rubber tree (Hevea brasiliensis), a member of the Euphorbiaceae family, is a perennial crop spreading over the whole tropical belt of the world, which is almost the sole source of natural rubber (NR). NR is a latex polymer with high elasticity, flexibility, and resilience that plays an important role in the world economy, and has not been totally replaced by synthetic rubber. NR has been used in industry, agriculture and national defense, etc., and its need is increasing every year (Lieberei 2007). Rubber anthracnose caused by Colletotrichum gloeosporioides is the main factor for the decline in the yields of NR. The pathogen infects most organs of rubber tree, resulting in anthracnose on leaves, twigs, flowers and young fruits. The consequences of the disease are leaf necrosis, deformation and defoliation, resulting in a decrease in rubber production (Jean et al. 2005). C. gloeosporioides can infect during different stages of plant growth with various infection modes, and it is currently difficult to control Communicated by Olaf Kniemeyer. * Xiao‑Yu Li [email protected] 1
Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China
the pathogen (Cai et al. 2013). Therefore, understanding the biological function of the genes related in pathogenic progress will contribute to knowing the molecular pathogenicity mechanisms with the impact on the control of C. gloeosporioides. G-protein signaling pathways are conserved in all eukaryotes and are crucial components sensing and relaying external cues into the cells to elicit appropriate physiological and
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