Gastroprotective effects of extracts and guttiferone A isolated from Garcinia achachairu Rusby (Clusiaceae) against expe
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ORIGINAL ARTICLE
Gastroprotective effects of extracts and guttiferone A isolated from Garcinia achachairu Rusby (Clusiaceae) against experimentally induced gastric lesions in mice Rivaldo Niero & Marlova Manhabosco Dal Molin & Suellen Silva & Natália Santos Damian & Láis Orlof Maia & Franco Delle Monache & Valdir Cechinel Filho & Sérgio Faloni de Andrade
Received: 2 March 2012 / Accepted: 17 July 2012 / Published online: 29 August 2012 # Springer-Verlag 2012
Abstract This study was undertaken to evaluate the gastroprotective properties of seed, leaf, and branch methanolic extracts and guttiferone A obtained from Garcinia achachairu (Clusiaceae). Mice were used in all the models, and treatments were administered orally only in pylorus-ligated model of the extracts, and drugs were administered intraduodenally. Treatment with different extracts (500 mg/kg) significantly reduced the ulcerative lesions in the ethanol/ HCl-induced model; however, the seed extract was most active. When tested in different doses (50, 250, or 500 mg/ kg), the seed extract of G. achaicharu showed a dosedependent effect with a percentage of inhibition of gastric lesions of 41, 49, and 85 %, respectively. The seed extract also significantly reduced the ulcerative lesions in the indomethacin/bethanechol-induced ulcer. In this model, the percentage of inhibition of ulcer was 24, 58, and 90 %, respectively. Regarding the model of gastric secretion, a reduction of gastric juice volume and total acidity was observed, as well as an increase in gastric pH. Considering that the seed extract was the most active, it was subjected to silica gel column chromatography, leading to the isolation of guttiferone A. The isolated compound and omeprazole were evaluated in the HCl/ethanol-induced ulcer model. In this assay, both compounds at a dose of 30 mg/kg reduced the ulcerative lesions by about 75 %. These results demonstrate, for the first time, that extracts obtained from G. achachairu R. Niero : M. M. D. Molin : S. Silva : N. S. Damian : L. O. Maia : F. D. Monache : V. C. Filho : S. F. de Andrade (*) Programa de Mestrado em Ciências Farmacêuticas, Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí–UNIVALI, Rua Uruguai, 458, Itajaí, Santa Catarina, Brazil e-mail: [email protected]
and guttiferone A produce gastroprotective effects, corroborating ethnomedicinal use of this plant. Keywords Garcinia achachairu . Guttiferone A . Gastroprotective action . Ulcer
Introduction Gastric hyperacidity and gastroduodenal ulcer occur in all ages and are among the most common ailments today. These diseases are of multifactorial etiology, and their pathophysiology is not yet completely understood. However, it is believed that gastric or duodenal ulcers are the result of an imbalance of aggressive and defensive gastric luminal factors. The main aggressive factors are acid, pepsin, Helicobacter pylori, and bile salts. Defensive factors mainly involve mucus–bicarbonate secretion and prostaglandins (Hoogerwerf and Pasricha 2001). This im
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