Characterization and Antibacterial Activity Against Helicobacter pylori of Lactic Acid Bacteria Isolated from Thai Ferme
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Characterization and Antibacterial Activity Against Helicobacter pylori of Lactic Acid Bacteria Isolated from Thai Fermented Rice Noodle Sujitra Techo 1 & Wonnop Visessanguan 2 & Ratha-korn Vilaichone 3 & Somboon Tanasupawat 1
# Springer Science+Business Media, LLC, part of Springer Nature 2018
Abstract A total of 32 lactic acid bacteria (LAB) were isolated from Khanom-jeen, a Thai traditional fermented rice noodle. They belonged to the genus Leuconostoc (Ln), Lactobacillus (Lb), Enterococcus (E), Lactococcus (Lc), and Weissella (W), based on their phenotypic characteristics and 16S rRNA gene sequence analyses. The strains were identified as Ln. pseudomesenteroides (group 1, two strains), Ln. citreum (group 2, three strains), Ln. lactis (group 3, three strains), Lb. paracasei subsp. tolerans (group 4, two strains), E. faecium (group 5, three strains), Lc. lactis subsp. lactis (group 6, one strain), W. confusa (group 7, six strains), Lb. fermentum (group 8, seven strains), and Lb. plantarum subsp. plantarum and Lb. pentosus (group 9, five strains). Fifteen strains exhibited the inhibitory activity against Helicobacter pylori clinical isolates by spot-on-lawn method. Lb. fermentum P43-01 resisted to bile acids showed the broad spectrum of antimicrobial activity against H. pylori strains MS83 and BK364. These antagonistic effects were associated with proteinaceous compounds which are sensitive to α-chymotrypsin and pepsin. Results indicated that production of bacteriocin-like substances of selected strain might be the significant mechanism that exerted the inhibition on H. pylori. A potential strain could be used as probiotics in alternative or adjunctive therapy for a patient suffering from H. pylori infection. Keywords Antimicrobial activity . Fermented rice noodle . Helicobacter pylori . Lactic acid bacteria . Bacteriocins
Introduction Helicobacter pylori strain has been first successfully cultivated by Warren and Marshall in 1983 [1]. This bacterium is now recognized as a major cause of chronic gastritis and peptic ulcer and is a risk factor for gastric carcinoma [2]. H. pylori strains were found in the human stomach of about half of the world populations. The prevalence of infection of developing countries was higher (> 80%) than the developed ones (< 40%) [3]. Currently, the standard triple and sequential therapies have been
* Somboon Tanasupawat [email protected] 1
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand
2
National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phahonyothin Road, Pathum Thani 12120, Thailand
3
GI Unit, Department of Medicine, Thammasat University Hospital, Pathum Thani 12120, Thailand
widely used for H. pylori eradication. These therapies required proton pump inhibitor (PPI) plus antibiotics including amoxicillin, metronidazole, tinidazole, and clarithromycin [4]. However, antibiotic-based therapy had the significant effect on the composition of intesti
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