Antimicrobial activity of Lavandula stoechas phenolic extracts against pathogenic bacteria isolated from a hospital in M
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RESEARCH ARTICLES
Antimicrobial activity of Lavandula stoechas phenolic extracts against pathogenic bacteria isolated from a hospital in Morocco Yassine Ez zoubi1,2 · Abdellah Farah2 · Hicham Zaroual2 · Abdelhakim El Ouali Lalami2,3 Received: 10 May 2020 / Revised: 24 August 2020 / Accepted: 30 August 2020 © Society for Plant Research 2020
Abstract The objective of this study was to determine the chemical families and the total phenolic content in a hydro-ethanolic extract of Lavandula stoechas, as well as to evaluate the antibacterial effect of the different phenolic fractions of this plant against pathogenic bacterial strains isolated from a hospital in the city of Fez, Morocco. The hydro-ethanolic extract was obtained and phytochemical screening of the different chemical families of the plant was conducted using colorimetric methods. The quantification of the total concentration of polyphenols in the L. stoechas extract was conducted according to the Folin–Ciocalteu method. The antimicrobial effects of the hydro-ethanolic, flavonoid and tannin extracts against Gram-positive and Gram-negative pathogenic bacteria isolated from a hospital in Fez were determined using the disc diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined for all pathogenic and reference strains. The total phenolic content of L. stoechas was 130.15 ± 3.72 mg gallic acid equivalent/g plant extract. The total phenolic content of L. stoechas was 130.15 ± 3.72 mg gallic acid equivalent/g plant extract. The flavonoid extract had the highest effect against bacteria, with MIC and MBC values varying from 10 to 40 mg/ml and 20–80 mg/ml, respectively. The results also revealed that the hydro-ethanolic extract showed a MIC value of 80 mg/ml against all bacteria except Acinetobacter baumanii and Pseudomonas aeruginosa (ATCC27853). The results obtained in our study showed an important antibacterial effect of flavonoid extracts from L. stoechas compared to other extracts. These results can aid in the discovery of new antimicrobial molecules of natural origin. Further research on phenolic compounds in L. stoechas is required. Keywords Lavandula stoechas · Ultrasound assisted extraction · Phenolic fractions · Antimicrobial activity · Hospital environment
Introduction An infection is said to be nosocomial if it occurs during or following the treatment (diagnostic, therapeutic, palliative, preventive or educational) of a patient in a health care
* Yassine Ez zoubi [email protected] 1
Biotechnology, Environmental Technology and Valorization of Bio‑Resources Team, Department of Biology, Faculty of Science and Technology Al‑Hoceima, Ajdir 32003, Abdelmalek Essaadi University, Tetouan, Morocco
2
Laboratory of Applied Organic Chemistry, Faculty of Sciences and Technology, Sidi Mohamed Ben Abdellah University, route D’imouzzer, PO box 2202, Fez, Morocco
3
Higher Institute of Nursing Professions and Health Techniques of Fez, Regional Health Directorate Fez-Meknes, El Ghassani
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