Gelatinase (MMP-2 and -9) expression profiles during gestation in the bovine endometrium
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Gelatinase (MMP-2 and -9) expression profiles during gestation in the bovine endometrium Keiichiro Kizaki†1, Koichi Ushizawa†2, Toru Takahashi2, Osamu Yamada3, Junichi Todoroki4, Takashi Sato5, Akira Ito5 and Kazuyoshi Hashizume*1 Address: 1Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Iwate University, Ueda 3-18-8, Morioka, Iwate 020-8550, Japan, 2Department of Developmental Biology, National Institute of Agrobiological Sciences, Ikenodai 2, Tsukuba, Ibaraki 305-8602, Japan, 3Miyagi Prefectural Animal Health Hygiene Ogawara Station, Miyagi 989-1243, Japan, 4Team Todoroki ARR, Kurahara, Miyakonojo, Miyazaki 855-0051, Japan and 5Department of Biochemistry and Molecular Biology, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 1920392, Japan Email: Keiichiro Kizaki - [email protected]; Koichi Ushizawa - [email protected]; Toru Takahashi - [email protected]; Osamu Yamada - [email protected]; Junichi Todoroki - [email protected]; Takashi Sato - [email protected]; Akira Ito - [email protected]; Kazuyoshi Hashizume* - [email protected] * Corresponding author †Equal contributors
Published: 31 December 2008 Reproductive Biology and Endocrinology 2008, 6:66
doi:10.1186/1477-7827-6-66
Received: 25 October 2008 Accepted: 31 December 2008
This article is available from: http://www.rbej.com/content/6/1/66 © 2008 Kizaki et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: Various molecules participate in implantation and maintaining endometrial function during gestation. The remodeling of endometrial matrices is a necessary process in the coordination of gestational progress. Matrix-metalloproteinases (MMPs) like gelatinases (MMP-2 and -9) and collagenase (MMP-1) are considered to play important roles in this process. We examined MMP-2 and -9 expression using zymography, in situ hybridization, real-time PCR, and microarray analysis to clarify their roles in the bovine endometrium during gestation. Methods: Endometria, placentomes, and fetal membranes were collected from Japanese black cows that were killed on day 15 to 252 of gestation or during their estrous cycle. The gene expression of MMP-related molecules (mainly MMP-2 and -9) was examined using a custom-made microarray, real-time RT-PCR, and in-situ hybridization. Gelatinase activity was detected by zymography and film in situ zymography. Results: Both gelatinases were expressed in the endometrium and fetal tissues throughout gestation. MMP-2 gene expression declined with the progress of gestation, but its intensity was maintained at a high level during the peri-implantation period and increased in late gestation. The expression level of MMP-9 was stably maintained, but was relatively low
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