TLR3 and TLR4 expression in healthy and diseased human endometrium
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TLR3 and TLR4 expression in healthy and diseased human endometrium Svenja Allhorn†1, Carsten Böing†2, Andrea A Koch1, Rainer Kimmig and Isabella Gashaw*1 Address: 1Institute of Anatomy II, University of Duisburg-Essen, Hufelandstr. 55, 45147 Essen, Germany and 2Department of Obstetrics and Gynaecology, University of Duisburg-Essen, Hufelandstr. 55, 45147 Essen, Germany Email: Svenja Allhorn - [email protected]; Carsten Böing - [email protected]; Andrea A Koch - [email protected]; Rainer Kimmig - [email protected]; Isabella Gashaw* - [email protected] * Corresponding author †Equal contributors
Published: 7 September 2008 Reproductive Biology and Endocrinology 2008, 6:40
doi:10.1186/1477-7827-6-40
Received: 10 April 2008 Accepted: 7 September 2008
This article is available from: http://www.rbej.com/content/6/1/40 © 2008 Allhorn et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: Toll-like receptors (TLRs) play an essential role in the innate immune system by initiating and directing immune response to pathogens. TLRs are expressed in the human endometrium and their regulation might be crucial for the pathogenesis of endometrial diseases. Methods: TLR3 and TLR4 expression was investigated during the menstrual cycle and in postmenopausal endometrium considering peritoneal endometriosis, hyperplasia, and endometrial adenocarcinoma specimens (grade 1 to 3). The expression studies applied quantitative RT-PCR and immunolabelling of both proteins. Results: TLR3 and TLR4 proteins were mostly localised to the glandular and luminal epithelium. In addition, TLR4 was present on endometrial dendritic cells, monocytes and macrophages. TLR3 and TLR4 mRNA levels did not show significant changes during the menstrual cycle. In patients with peritoneal endometriosis, TLR3 and TLR4 mRNA expression decreased significantly in proliferative diseased endometrium compared to controls. Interestingly, ectopic endometriotic lesions showed a significant increase of TLR3 und TLR4 mRNA expression compared to corresponding eutopic tissues, indicating a local gain of TLR expression. Endometrial hyperplasia and adenocarcinoma revealed significantly reduced receptor levels when compared with postmenopausal controls. The lowest TLR expression levels were determined in poor differentiated carcinoma (grade 3). Conclusion: Our data suggest an involvement of TLR3 and TLR4 in endometrial diseases as demonstrated by altered expression levels in endometriosis and endometrial cancer.
Background Toll-like receptors (TLRs) recognize specific pathogen associated molecular patterns (PAMPs) and serve an essential role in the innate immune system by initiating and directing immune response to microbial pathogens.
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