Generation and molecular characteristics of a highly attenuated GPV strain through adaptation in GEF cells
- PDF / 1,035,184 Bytes
- 7 Pages / 595.276 x 790.866 pts Page_size
- 89 Downloads / 174 Views
RESEARCH ARTICLE
Open Access
Generation and molecular characteristics of a highly attenuated GPV strain through adaptation in GEF cells Hongxia Shao1,2,3,4*†, Yuchen Jiang1,2,3,4†, Huisha Yuan1,2,3,4, Lifei Ji1,2,3,4, Wenjie Jin1,2,3,4, Kun Qian1,2,3,4, Jianqiang Ye1,2,3,4 and Aijian Qin1,2,3,4*
Abstract Background: Goose parvoviruses (GPVs) spread globally and cause a huge economic loss to the poultry industry. Although the attenuated GPV vaccines play a key role in preventing the disease caused by GPV, the molecular basis for the attenuation of GPV is barely known. Results: A highly attenuated GPV strain, GPV-CZM-142, was generated through blindly passaging of the highly pathogenic strain, GPV-CZM, in goose embryonic fibroblasts (GEF) for 142 generations. The GEF-adapted GPV strain’s virulence was 10,000 times weaker than its wild type counterpart, GPV-CZM, based on the ELD50 (50% Embryo Lethal Dose). By comparing with the wild type strain, genome sequencing analysis identified adapted mutations either in ITR or in NS and VP1 of GPV-CZM-142. Conclusions: The highly attenuated GPV strain, GPV-CZM-142, provides a GPV vaccine candidate, and the identified virulence-related mutations give a novel insight into the molecular determinants of GPV virulence. Keywords: Goose parvovirus, Attenuated virus, Gene mutation, Vaccine, Virulence
Background Goose parvovirus (GPV) belongs to the genus parvoviruses, family Parvoviridae. GPV is a non-enveloped and single-stranded DNA virus. The genome of GPV contains two inverted terminal repeats (ITRs) and two major open reading frames (ORFs). ITR carries important elements for viral replication and encapsidation. The two major ORFs encode the non-structural protein (NS) and the capsid proteins (VP1/2/3). VP1 is the largest capsid protein, which shares a common region of Cterminus with VP2 and VP3. As a globally spread virus, * Correspondence: [email protected]; [email protected] † Hongxia Shao and Yuchen Jiang contributed equally to this work. 1 Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine, Yangzhou University, No.12 East Wenhui Road, Yangzhou 225009, Jiangsu, China Full list of author information is available at the end of the article
GPV was first isolated and identified in 1956 by Fang in China [1, 2]. Its infection mainly causes acute contagious and septic diseases in goslings and Muscovy ducks with age younger than 30 d old [3]. The intestinal suppository is a characteristic lesion for GPV infection in goslings [4]. Although the attenuated GPV vaccine and its yolk antibody play a key role in preventing disease deterioration caused by GPV, GPV is still frequently isolated in the GPV-vaccinated goose. Also, the current strategies for GPV prevention and control are challenged with the following problems: later onset of GPV infection in goose/duck individuals, more emergence of the GPV mutants, and the co-infection of GPV with other pathogens (e.g., goose astrovirus
Data Loading...