Genetic analysis of anther culture response and identification of QTLs associated with response traits in wheat ( Tritic
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ORIGINAL ARTICLE
Genetic analysis of anther culture response and identification of QTLs associated with response traits in wheat (Triticum aestivum L.) Bahaa E. S. Abd El‑Fatah1 · Mohammed A. Sayed2 · Sahar A. El‑Sanusy1 Received: 8 May 2020 / Accepted: 16 November 2020 / Published online: 23 November 2020 © Springer Nature B.V. 2020
Abstract Anther culture is the most effective tool for doubled haploid production of wheat. This investigation was conducted to estimate genetic parameters of anther culture response in wheat and identification of putative Quantitative trait loci (QTLs) associated with response traits. Two varieties of wheat, namely ICR-DH ( P1) and Sle 1 × 15 ( P2) and their F 1 and F 2 progenies were used in the present investigation to estimate genetic parameters of anther culture response. Two molecular marker systems, SRAP and SSR markers were used to detect the polymorphism between two anther donor parents. Single marker analysis (SMA) and Composite interval mapping (CIM) were used to localize the putative QTL associated with four anther culture response in wheat using 100 plants of F2 population derived from F1 cross ‘ICR-DH’ × ‘Sel 1 × 15’. Analyses of variance indicated significant differences between four populations ( P1, P2, F1 and F2) for callus induction (CAL), number of green plants per 100 anther (GR), number of albino plant per 100 anther (AR) and total regenerated plants per 100 anther (TR). The additive effects were more important than dominance effects in controlling these traits. The two molecular marker systems were sufficient in detecting polymorphism between two parents. Thirty two putative QTLs were detected on eight linkage groups. Our study indicated that the additive effects of genes and detection of new QTLs permit marker-assisted selection of genotypes with high green plantlet regeneration efficiency in anther culture, and therefore favor efficient use of anther culture in wheat breeding programs Keywords Wheat · Anther culture · SSR · SRAP · SMA · QTL
Introduction Anther culture technique in wheat and other important cereal crops is one of the fastest and simplest methods to obtain haploid and doubled haploid plants (DHs), which are very Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11033-020-06007-z) contains supplementary material, which is available to authorized users. * Bahaa E. S. Abd El‑Fatah [email protected]; [email protected] Mohammed A. Sayed [email protected] Sahar A. El‑Sanusy [email protected] 1
Department of Genetics, Faculty of Agriculture, Assiut University, Assiut 71526, Egypt
Department of Agronomy, Faculty of Agriculture, Assiut University, Assiut 71526, Egypt
2
important paraphernalia for crop breeding programs, as well as for molecular and genetical studies [1]. However, the scanty androgenetic capability of numerous genotypes is still a problem that finite the use of this technique in wheat breeding [2]. A genotype is one of the most importa
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