Identification of QTLs associated with Sclerotinia blight resistance in peanut ( Arachis hypogaea L.)
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RESEARCH ARTICLE
Identification of QTLs associated with Sclerotinia blight resistance in peanut (Arachis hypogaea L.) Yuya Liang
. John M. Cason . Michael R. Baring . Endang M. Septiningsih
Received: 14 June 2020 / Accepted: 1 September 2020 Ó Springer Nature B.V. 2020
Abstract Sclerotinia blight caused by Sclerotinia minor (Jagger) is a significant threat to peanut production; therefore varietal improvement toward this disease is needed. To date, there have been no reported quantitative trait locus (QTL) associated with Sclerotinia blight resistance in peanut. Hence, the objective of this study was to identify QTLs for Sclerotinia blight resistance. A total of 90 F2:6 recombinant inbred lines, derived from a released cultivar Tamrun OL07 and a breeding line Tx964117, were used as mapping population and field experiments were conducted in 2010, 2012 and 2018 at the Texas A&M AgriLife Research and Extension Center at Stephenville, Texas. A genetic map was developed using 1211 SNP markers based on double digest restriction-site associated DNA sequencing (ddRADSeq). In total, seven QTLs were identified, two QTLs from 2010 and five QTLs from 2018, with LOD score values of 3.2 to 7.2 and explaining 6.6–25.6% Y. Liang M. R. Baring E. M. Septiningsih (&) Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA e-mail: [email protected] Present Address: Y. Liang Department of Plant Biology, Michigan State University, East Lansing, MI 48824, USA J. M. Cason Stephenville Research Center, Texas A&M AgriLife Research, Stephenville, TX 76401, USA
phenotypic variance. Among these QTLs, three were detected in common by WinQTLCart and R/qtl. Interestingly, one of the QTLs coincides with a previously reported peanut Leaf spot resistance QTL. The findings from this study not only provide insights into disease resistant QTLs in peanut but can also be used as potential targets for breeding programs to enhance Sclerotinia blight resistance through molecular breeding. Keywords QTL Disease ddRAD-seq Sclerotinia blight Peanut (Arachis hypogea L.)
Introduction Peanut (Arachis hypogaea L.) accounts for more than $1.6 billion in value in the United States and is also the third most widely grown oilseed crop in the world. Peanut seeds have high nutritional values, with high oil (40-60%) and protein (20–30%) contents (Mallikarjuna and Varshney 2014). A soil-borne fungal disease Sclerotinia blight, caused by Sclerotinia minor, is one of the most destructive plant pathogens worldwide. S. minor is capable of infecting around one hundred host plants and causes substantial damage and yield reduction of 10% to 75% of various crops such as lettuce, chicory, green bean, sunflower, and peanut (Melzer et al. 1997). S. minor was first identified on
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peanut in Australia in 1948, was first reported in the United States in Virginia in 1971, and the first outbreak in Texas was in 1981 (Crutcher et al. 2018; Goldman et al. 1995
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