Genetic Analysis of Chelonid Herpesvirus 5 in Marine Turtles from Baja California Peninsula
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Ó 2020 EcoHealth Alliance
Short Communication
Genetic Analysis of Chelonid Herpesvirus 5 in Marine Turtles from Baja California Peninsula Joelly Espinoza,1 Elsa Herna´ndez,1 Marı´a Mo´nica Lara-Uc,2,3 Eduardo Rese´ndiz,2,3 Alonzo Alfaro-Nu´n˜ez,4,5 Sawako Hori-Oshima,1 and Gerardo Medina-Basulto1 1
Laboratorio de Biologı´a Molecular, Instituto de Investigaciones en Ciencias Veterinarias, Universidad Auto´noma de Baja California, Campestre Ave. W/N Fracc. Laguna Campestre, 21380 Mexicali, Baja California, Mexico 2 Laboratorio de Oceanografı´a y Bota´nica Marina, Departamento Acade´mico de Ciencias Marinas y Costeras, Universidad Auto´noma de Baja California Sur, 23080 La Paz, Baja California Sur, Mexico 3 Proyecto Salud de Tortugas Marinas, Laboratorio de Oceanografı´a, Universidad Auto´noma de Baja California Sur, 23080 La Paz, Baja California Sur, Mexico 4 Virus Research & Development Laboratory (ViFU), Department of Virus and Microbiological Special Diagnostics, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark 5 Section for Evolutionary Genomics, Centre for GeoGenetics, Natural History Museum of Denmark, University of Copenhagen, 1350 Copenhagen, Denmark
Abstract: The Chelonid herpesvirus 5 (ChHV5) is the primary etiological agent associated with fibropapillomatosis (FP), a neoplastic disease in marine turtles. In this study, we report for the first time ChHV5 in marine turtles and a leech from Baja California Peninsula. Eighty-seven black, olive or loggerhead turtle species, one FP tumor and five leeches were analyzed. The tumor sample from an olive, a skin sample from a black and a leech resulted positive of ChHV5 for conventional PCR. Two viral variants were identified and grouped within the Eastern Pacific phylogenetic group, suggesting a possible flow of the virus in this region. Keywords: Fibropapillomatosis, PCR identification, Chelonid herpesvirus 5, Marine turtles, Eastern Pacific, Phylogenetic analysis
Fibropapillomatosis (FP) is a debilitating neoplastic disease affecting marine turtles worldwide and is characterized by the presence of epithelial fibropapillomas and internal fibromas (Herbst 1994; Quackenbush et al. 2001). Originally described in green turtles (Chelonia mydas), FP has
Electronic supplementary material: The online version of this article (https://doi. org/10.1007/s10393-020-01482-z) contains supplementary material, which is available to authorized users.
Correspondence to: Gerardo Medina-Basulto, e-mail: [email protected]
subsequently been documented among all seven species of marine turtles (Herbst 1994; Barragan and Sarti 1994; Lackovich et al. 1999; Herbst et al. 2004; Ene et al. 2005; Williams et al. 2006; Work et al. 2009; Alfaro-Nu´n˜ez and Gilbert 2014) in all oceans (Alfaro-Nu´n˜ez et al. 2014) and thus has a circumtropical distribution (Aguirre and Lutz 2004). FP has been linked to the presence of the Chelonid herpesvirus 5 (ChHV5) (Jacobson et al. 1991; Quackenbush et al. 1998). ChHV5 has been detected in different regions and species of marine tur
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