Genetic copy number alterations and IL-13 expression differences in papillary thyroid cancers and benign nodules
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ORIGINAL PAPER
Genetic copy number alterations and IL-13 expression differences in papillary thyroid cancers and benign nodules ZeFei Zhao Æ Qing Wei Æ YongJu Zhao Æ Fukang Sun Æ Xiaolong Jin Æ Bin Cui Æ Guang Ning
Received: 27 February 2009 / Accepted: 15 May 2009 / Published online: 9 June 2009 Ó Humana Press 2009
Abstract Thyroid nodules were the extremely common endocrine tumors, in which papillary thyroid carcinomas (PTCs) were the most prevalent endocrine malignancy, representing 80–90% of all thyroid malignancies. It was still a dilemma to discriminate PTCs and benign thyroid nodules. With a new molecular genetics technology of Multiplex ligation-dependent probe amplification (MLPA), we investigated 13 PTC and 14 benign nodule tissue samples. The results showed that PTCs had more genetic copy number alteration than benign nodules (P \ 0.001). Receiver operating characteristic (ROC) curve analysis suggested that genomic aberrations would provide a moderate accuracy method to discriminate PTCs and benign nodules. The gain of interleukin 13 (IL-13) gene obviously identified the great difference between PTCs and benign nodules. Immunohistochemistry also confirmed
ZeFei Zhao and Qing Wei contributed equally to this work. Z. Zhao Y. Zhao F. Sun B. Cui (&) G. Ning Shanghai Key Laboratory for Endocrine Tumors, Shanghai Clinical Center for Endocrine and Metabolic Diseases, Shanghai Institute of Endocrinology and Metabolism, Ruijin Hospital, Shanghai JiaoTong University School of Medicine, 197 RuiJin Er Lu, Shanghai 200025, People’s Republic of China e-mail: [email protected] Q. Wei X. Jin Department of Pathology, Ruijin Hospital, Shanghai JiaoTong University School of Medicine, 197 RuiJin Er Lu, Shanghai 200025, People’s Republic of China B. Cui G. Ning Laboratory Endocrine & Metabolic Diseases of Institute of Health Science, Shanghai JiaoTong University School of Medicine and Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 225 South ChongQing Road, Shanghai 200025, People’s Republic of China
significantly higher IL-13 expression in the PTCs (P \ 0.001). The current study showed that MLPA should be an effective method to diagnose PTCs and benign thyroid nodules, and also provided a clue to another relationship between IL-13 and PTCs. Keywords Papillary thyroid carcinomas (PTCs) IL-13 Multiplex ligation-dependent probe amplification (MLPA)
Introduction Thyroid nodules were the extremely common endocrine and 5–10% of the population would develop a clinically significant thyroid nodule during their life [1]. However, only about 5% clinically identified nodules were malignant [2]. Papillary thyroid carcinomas (PTCs) were the most prevalent endocrine malignancy, representing 80–90% of all thyroid malignancies [3]. Fine needle aspiration biopsy (FNA) was the best test to evaluate a patient with a benign or malignant thyroid nodule. However, 10–25% of FNAs were not definitively diagnosed as benign or malignant [4]. During the past decades, significant progress has been made in the
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