Genetic Dissection of Component Traits for Salinity Tolerance at Reproductive Stage in Rice
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ORIGINAL ARTICLE
Genetic Dissection of Component Traits for Salinity Tolerance at Reproductive Stage in Rice Krishnendu Chattopadhyay 1 & Sangram Keshori Mohanty 1 & Joshitha Vijayan 2 & Bishnu Charan Marndi 1 & Ananta Sarkar 3 & Kutubuddin Ali Molla 1 & Koushik Chakraborty 1 & Soham Ray 4 & Ramani Kumar Sarkar 1 Received: 19 November 2019 / Accepted: 29 October 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Rice is highly sensitive to salt stress at flowering stage. With the objective of detection of quantitative trait loci (QTLs) in multienvironment for this stage, 180 backcross-derived lines (BC3F5) from salt tolerant donor Pokkali (AC41585) and recurrent parent IR 64 were subjected to evaluation in saline (EC = 8 dSm−1) and non-saline environments in wet season of 2014 and 2015 employing a novel phenotyping protocol. Nine multi-environmental consistent QTLs for spikelet degeneration, K+ concentration in flag leaf, stress susceptibility index for grain (SSI-Grain) and spikelet sterility (SSI-STE) on chromosomes1, 2, 3, 4 and 11 with 17–42% phenotypic variances were detected. Among several digenic epistatic interactions, one was associated with the main effect QTL (qSSI-STE-11-1) over the years. Similarly genotype × environment interaction associated with two additive QTLs, qDEG-S-2-2 and qSSI-STE-2-1 had positive effect on the resultant phenotype. Functional genes encoding calmodulin-binding protein and potassium transporter were predicted inside the consistent QTLs. Detected stable QTLs, associated markers, predicted genes and derived introgression lines with these QTLs could be utilized in future breeding programme. Keywords Salt stress . Flowering stage . Quantitative trait loci . Genotype × environment interaction . Epistatic . Rice
Introduction Salinity is now becoming a wide spread problem for rice cultivation in India and other rice-growing countries in the world Key message • Employing a population using salt tolerant donor at reproductive stage, Pokkali (AC 41585), nine multi-environmental consistent QTLs for spikelet degeneration, spikelet sterility, K+ concentration at flag leaf, etc. were discovered. • Two genotype × environment interaction QTLs and one digenicepistatic QTL were detected with synergistic effect on the main effect QTLs. * Krishnendu Chattopadhyay [email protected] 1
ICAR-National Rice Research Institute, Cuttack, Odisha, India
2
ICAR- Central Island Agricultural Research Institute, Port Blair, Andaman and Nicobar Island, India
3
ICAR-Central Institute for Women in Agriculture, Bhubaneswar, Odisha, India
4
ICAR-Central Research Institute for Jute and Allied Fibres, Barrackpore, West Bengal, India
(Hossain et al. 2015). Rice is mainly susceptible to salt stress at early vegetative and reproductive stages. More than 100 quantitative trait loci (QTLs) for salt stress tolerance have been mapped in all 12 chromosomes of rice, mainly for the seedling stage (Kumar et al. 2015). From Pokkali, one QTL, named Saltol, was detected and incorpor
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