Genetic homogeneity assessment of in vitro -regenerated plantlets of Nyctanthes arbor-tristis L. and comparative evaluat
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PLANT TISSUE CULTURE
Genetic homogeneity assessment of in vitro-regenerated plantlets of Nyctanthes arbor-tristis L. and comparative evaluation of bioactive metabolites and antioxidant activity Sobha Chandra Ratha 1 & Sen Seth 1 & Sujit K. Mishra 1 & Pawan K. Yadav 2 & Aditya K. Gupta 2 & Jogeswar Panigrahi 1,2,3 Received: 8 May 2018 / Accepted: 4 July 2019 / Editor: Pamela Weathers # The Society for In Vitro Biology 2019
Abstract In vitro propagation of Nyactanthes arbor-tristis L. was achieved by culturing N-phenyl-N′-benzothiazol-6-yl-urea (PBU)pretreated nodal explants in Murashige and Skoog (MS) medium without any phytohormones. Pretreatment of nodal explants in liquid MS medium with 100 μM N-phenyl-N′-benzothiazol-6-yl-urea for 4 d showed the highest shoot proliferation by producing maximum number of shoots (17.40 ± 1.02) per explant, with average shoot length of 5.96 ± 0.08 cm at the end of 8 wk. Effective rooting was accomplished by preincubating the cut-end of shoots with half-strength MS medium containing 6 μM indole-3-butyric acid for 1 wk, followed by implantation into half-strength MS medium; an average of 6.20 ± 0.049 roots per shoot were produced. Seventy-eight percent of the plantlets regenerated in vitro were successfully acclimatized and transferred to soil. These plantlets appeared to be morphologically similar to the donor plants. The genetic fidelity of these in vitroregenerated plantlets was confirmed by start codon targeted polymorphism (SCoT) marker analysis, followed by comparative evaluations of the bioactive metabolites (ursolic acid, rengyolone, arbortristoside-A, and nyctanthoside), antioxidant-rich phytochemicals, and radical scavenging activities. This optimized in vitro propagation protocol should be an aid for the conservation of N. arbor-tristis germplasm, as well as cater to the needs of herbal industries for the production of therapeutic molecules. Keywords Antioxidant activity . Bioactive metabolites (arbortristoside-A, nyctanthoside, rengyolone, and ursolic acid) . N-Phenyl-N′-benzothiazol-6-yl-urea (PBU) . Start codon targeted polymorphism (SCoT) marker analysis
Introduction Nyctanthes arbor-tristis L. (family Oleaceae), commonly known as “night flowering jasmine,” is an important medicinal species. Although this plant is native to the Indian subcontinent, where it is distributed in the wild in sub-Himalayan regions, night flowering jasmine is also grown as an ornamental in subtropical gardens to cater to ritual and esthetic needs. * Jogeswar Panigrahi [email protected] 1
Plant Biotechnology Laboratory, School of Life Sciences, Sambalpur University, Jyoti Vihar, Sambalpur, Odisha 768019, India
2
Department of Biotechnology, School of Life Sciences, Central University of Rajasthan, NH-8 Bandarsindri, Kishangarh, Rajasthan 305817, India
3
Present address: Department of Bioscience and Bioinformatics, Khallikote University, Berhampur, Odisha 760001, India
This plant is a promising source of many therapeutic biomolecules, such as the iridoid glycosides arbortristoside
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