Assessment of Genetic Diversity in Some Indian Lablab purpureus , L. Bean Genotypes Based on RAPD Marker
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RESEARCH ARTICLE
Assessment of Genetic Diversity in Some Indian Lablab purpureus, L. Bean Genotypes Based on RAPD Marker Vishwajeet Singh1 • Rajdeep Kudesia1 • Seema Bhadauria2
Received: 13 February 2018 / Revised: 11 November 2019 / Accepted: 6 December 2019 Ó The National Academy of Sciences, India 2019
Abstract In the present study, ten genotypes of Lablab purpureus L. Sweet bean were screened for genetic divergence by using 5 primers, i.e., OPA 01, OPA 04, OPA 13, OPB 09, and OPB 10. Analysis of diversity such as PIC, MI, MRP, Dendrogram, and RP was done. Out of the 5 primers used, the average number of Polymorphic bands detected was 7.6 and the average level of polymorphism was 74.23%. OPA 01 showed the highest level of polymorphism (92.85%) while OPB-09 showed the least level of polymorphism, i.e., 55.55%. The polymorphic information content ranged from 0.24 (OPA-04) to 0.318 (OPA01) at an average PIC value of 0.275. The mean of resolving power per RAPD primer is 3.65. The MI value ranged from 1.32 to 2.79 with a mean of 1.79/primer. The mean of MRP per primer was 0.47. The clustering of genotype was performed following the UPGMA method. The optimal tree with the sum of length branch is 13.30325. Keywords Lablab purpureus RAPD Genetic diversity Molecular markers
Significance Statement The genetic diversity study is useful in characterization genetic variability among varieties of Lablab purpureus for future research in the field of breeding and earning higher remuneration from genetically ascertained varieties. & Vishwajeet Singh [email protected] 1
Department of Botany, Bundelkhand University, Jhansi, U.P, India
2
Department of Botany, R.B.S. College, Agra, U.P, India
Introduction Dolichos lablab or Lablab purpureus is an inhabitant of Southeast Asia or India [1]. It is likely to be originated from Asia and has been used for planting for a long time. A wild variety of Lablab is grown in India. D. lablab was taken to the African tropical part and from there spread across Indonesia, Sudan, Caribbean, Malaysia, Papua New Guinea, Mainland China, Philippines, West Africa, Egypt, and South America. Flowers grow in clusters, on an inflorescence that is unbranched at an angle between the main stem and leaf. Based on the variety, the color of the flower may be white, blue, or purple. Seed pods are 5 cm [2] to 10 cm in length [3], flat, smooth, pointed, and contain 2 to 4 seeds. Seeds may be cream, white, pale brown, red, dark brown, black, or mottled depending on the variety. The stems are twining and Cylindrical, hairy or glabrous, often 2–3 m, but usually to 10 m long. Others are dwarf and bushy. Leaves are alternate and trifoliate, with leaflets ovate, measuring 5–14 cm 9 4–14 cm. On the other hand, very broad leaflets, lateral ones are lopsided, 7.5–15 cm long and nearly as broad, rather abruptly acuminate. Lablab purpureus has originated in India but very less work has been reported for the genetic divergence for improving yield. During the last 12 years, different molecular techniques have be
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