Genetic Variability and Structure of the Caucasian Shrew Sorex satunini in the North Caucasus according to the Variabili
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AL GENETICS
Genetic Variability and Structure of the Caucasian Shrew Sorex satunini in the North Caucasus according to the Variability of Microsatellite Loci V. V. Stakheeva, *, M. A. Makhotkina, S. A. Kornienkob, A. A. Makarikovb, N. V. Panasjuka, and V. N. Orlovc aFederal bInstitute
Research Center Southern Scientific Centre, Russian Academy of Sciences, Rostov-on-Don, 344006 Russia of Systematics and Ecology of Animals, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630091 Russia c Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, 119071 Russia *e-mail: [email protected] Received August 9, 2019; revised September 30, 2019; accepted October 31, 2019
Abstract—The variability of the microsatellite loci of the Caucasian shrew Sorex satunini in the North Caucasus was evaluated. It was shown that this species on this territory is represented by two discrete genetic lines associated with the basins of the Kuban and Terek rivers. The genetic diversity of the Caucasian shrew from the line of the Kuban River basin is characterized by a significantly higher polymorphism compared to the line from the Terek River basin. These differences are associated with both modern habitat conditions of S. satunini and the history of this species in the Western and Central Caucasus. Keywords: Caucasian shrew, North Caucasus, genetic polymorphism, microsatellites, isolation DOI: 10.1134/S1022795420080153
INTRODUCTION The Caucasian shrew Sorex satunini Ogn. is one of the regional species of the group of common shrews S. araneus, common in the Western Ciscaucasia, the Caucasus, the Transcaucasia, the Iranian Highlands, and the Asia Minor. The intraspecific variability of the Caucasian shrew, including its genetic polymorphism and structure, has been poorly studied. Currently, the known variability of the cytochrome b gene in this species cannot be used to assess population genetic differentiation due to the presence of two independent mitochondrial lines [1–3]. The analysis of the polymorphism of microsatellite loci had worked well for characterizing the metapopulation structure of individual taxa and their genetic subdivision. This is the most clearly shown by the example with anadromous fish species [4, 5], and it is also successfully used in study of mammals [6, 7]. In the present work we tried to use the material of the variability of microsatellite loci to assess the level and nature of the genetic polymorphism of the Caucasian shrew within the central and western parts of the northern macroslope of the Caucasus and in the Western Ciscaucasia.
MATERIALS AND METHODS The liver tissue of 46 individuals of the Caucasian shrew caught in nine localities of the steppes of the Western Ciscaucasia and the North Caucasus served as the material of the study (Table 1, Fig. 1). Total genomic DNA was isolated by the standard method of salt extraction with the destruction of proteins by proteinase K in the presence of SDS [8]. The total DNA content in the resulting preparations was evaluated on a Qubi
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