Genome Sequence Analysis of Clostridium tyrobutyricum , a Promising Microbial Host for Human Health and Industrial Appli
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Genome Sequence Analysis of Clostridium tyrobutyricum, a Promising Microbial Host for Human Health and Industrial Applications Tingting Liu1 · Liying Zhu2 · Zhengming Zhu3 · Ling Jiang3 Received: 1 April 2020 / Accepted: 21 August 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Clostridium tyrobutyricum is a promising microbial host for the anaerobic production of bio-based chemicals, especially butyric acid. At the same time, it also has great potential as a probiotic for the production of short-chain fatty acids in the intestines. However, due to the insufficient knowledge of the genetic characteristics of this organism, there has been little progress in its genetic engineering to date. Here, we present the complete genome sequence of C. tyrobutyricum L319, which consists of a circular chromosome and a plasmid with a G + C content of 31.69%, encompassing approximately 3.09 Mb with 3052 protein-coding genes. Functional gene annotation revealed better results than previous studies based on KEGG pathway classification. Furthermore, we obtained detailed functional characterization of 93 genes previously annotated as putative proteins. Genomic analysis revealed that this organism contains multiple genes encoding enzymes involved in the CRISPR-Cas systems, substrate utilization, isopeptide and ester bonds, transcriptional regulation, and oxidative stress. The safety evaluation at genetic level indicated that this organism does not possess transferable resistance genes, invasive defensive pathogenicity factors, or harmful enzymes. The genome sequence data analyzed in this study will be available for further research and will facilitate the further understanding and development of potential applications of C. tyrobutyricum.
Introduction Clostridium species are a group of anaerobic Gram-positive bacteria that can produce spores with strong resistance to external environmental stress. Among them, Clostridium tyrobutyricum is considered arguably the most commercially valuable microbial cell factory for the production of Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02175-0) contains supplementary material, which is available to authorized users. * Zhengming Zhu [email protected] * Ling Jiang [email protected] Liying Zhu [email protected] 1
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 210009, People’s Republic of China
2
College of Chemical and Molecular Engineering, Nanjing Tech University, Nanjing 210009, People’s Republic of China
3
College of Food Science and Light Industry, Nanjing Tech University, Nanjing 210009, People’s Republic of China
various chemicals. With pentoses or hexoses as the fermentation substrate, the main product is butyric acid, together with potentially valuable by-products such as acetic acid, carbon dioxide, and hydrogen [1]. This organism is widely used as a butyric acid production strain due to the higher butyric acid yield and p
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