The Draft Genome Sequence of Clostridium butyricum QXYZ514, a Potent Bacterium for Converting Glycerol into Fuels and Bi
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The Draft Genome Sequence of Clostridium butyricum QXYZ514, a Potent Bacterium for Converting Glycerol into Fuels and Bioproducts in the Waste‑Based Biorefinery Miaomiao Yang1 · Hossain M. Zabed1 · Junhua Yun1 · Guoyan Zhang1 · Xianghui Qi1 Received: 25 March 2020 / Accepted: 25 August 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract An anaerobic bacterium Clostridium buturicum QXYZ514 was isolated from a pond soil sample located neighboring to a biodiesel factory. This bacterium possesses excellent metabolic features for converting biodiesel-derived glycerol into various bioproducts, including 1,3-propanediol, butyrate, lactate, and acetate, and fuels, like ethanol and butanol. To further improve the yield of the target products and minimize the production of the by-products, the whole genome sequence of this multipurpose strain might provide necessary genetic information, and hence, the complete genome of QXYZ514 strain was sequenced using the PacBio RS II sequencing method. According to the complete genome sequence, the genome of QXYZ514 consisted of two circular chromosomes with a total of 4,636,461 bp, where GC content was found to be 28.76%. Major predicted features of the genome included a total of 4220 coding sequences (CDS), 87 tRNAs genes, and 36 rRNAs genes, which were annotated with the help of different databases for a better understanding of this strain. Six possible CRISPR components were also predicted in the genome. The exploration of the complete genome sequence of the QXYZ514 strain would have the potential to enrich the diversity of this species, and to recognize some significant hydrolytic enzymes, which could provide the references for overcoming the bottlenecks in the biorefinery usage of this bacterium in the valorization of biodiesel-derived glycerol.
Introduction The global demand for biodiesel production has been increased dramatically in recent years as attempts to replace portions of fossil fuels with renewable and eco-friendly fuels. It has driven into the generation of significant amounts of glycerol from the biodiesel industries, as roughly 10 tons of crude glycerol can be produced from every 100 tons of biodiesel produced via transesterification of vegetable oils or animal fats [1, 2]. This surplus amount of glycerol can be valorized through its bioconversion into value-added fuels and chemicals [3], such as 1,3-propanediol, butyrate, acetate, lactate, ethanol, and butanol by some anaerobic clostridial species. This bioconversion process is attractive for its environmental friendliness and low energy consumption [4,
* Xianghui Qi [email protected] 1
School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, Jiangsu, China
5]. In recent years, sustainable development is becoming increasingly important to mitigate the growing demand for energy and chemicals, as well as the concern with environmental problems [6]. Sustainable development has been widely approved by the policymakers in many countries.
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